The 19S regulatory particle (PA700) locates on either one or both ends of the 20S core particle of the 26S proteasome. It has 19 subunits including six AAA ATPases that associate with α subunits of the 20S proteasome to form the 26S proteasome. PA700 plays an essential role in preparing substrate proteins to be degraded by the 20S proteasome, including binding, deubiquitinating and unfolding polyubiquitinated proteins.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

Bovine 19S (PA700)

PA700

A1301

50 µg

900 kDa

Bovine

Bovine red blood cells

20 mM Tris, 20 mM NaCl, I mM EDTA, 5 mM βME, 10% Glycerol

See tube label

At least 90% by native-PAGE, see datasheet for gel image

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Datasheet， MSDS

N/A

(Click image to enlarge)

Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified Bovine 19S (PA700)

Coomassie-stained native-PAGELane 1: 5 µg purified Bovine 19S (PA700)

Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA700, the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Dry ice shipping

1. Waxman L, et al. (1987) J Biol Chem 262(6), 2451 – 2457.2. Kanayama HO, et al. (1992) Eur J Biochem 206(2), 567 – 578.3. Glickman MH, et al. (1998) Cell 94, 615 – 623.4. Da Fonseca PC, et al. (2008) J Biol Chem 283, 23305 – 23314.5. Kim HM, et al. (2011) Biochim Biophysica Acta 1809(2), 67 – 79.

Details

The 19S regulatory particle (PA700) locates on either one or both ends of the 20S core particle of the 26S proteasome. It has 19 subunits including six AAA ATPases that associate with α subunits of the 20S proteasome to form the 26S proteasome. PA700 plays an essential role in preparing substrate proteins to be degraded by the 20S proteasome, including binding, deubiquitinating and unfolding polyubiquitinated proteins.Images(Click image to enlarge) Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified Bovine 19S (PA700) Coomassie-stained native-PAGELane 1: 5 µg purified Bovine 19S (PA700) Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA700, the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

>>> 更多资讯详情请访问蚂蚁淘商城