Additional Information
Anti-GFP |
Anti-GFP (GF28R) Mouse Monoclonal Antibody |
Y1030 |
25 µg |
~55/25 kDa |
N/A |
Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates |
10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide) |
1 µg/µl |
WB (with ECL): 1-10 ng of purified GFP or GFP fusion proteins |
Store at -20°C or -80°C for one year. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. |
PDF Datasheet, MSDS |
N/A |
(Click image to enlarge)1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used |
Wet ice shipping |
N/A |
Details
SpecificityRecognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFPIsotypeMouse IgG2bCloneGF28RPurificationProtein A affinity chromatography from mouse ascites fluidApplicationsDot, ELISA, IP, IS, WBWorking ConditionsWB (with ECL): 1:1000-3000 dilutionIS: 1:500-2000 dilutionFor best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration testImages(Click image to enlarge)1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used>>> 更多资讯详情请访问蚂蚁淘商城