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ubpbio/Anti-GFP/Y1030/25 µg

作者: 时间:2024-11-14 点击量:

Anti-GFP (GF28R) Mouse Monoclonal Antibody

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:
Anti-GFP
Anti-GFP (GF28R) Mouse Monoclonal Antibody
Y1030
25 µg
~55/25 kDa
N/A
Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)
1 µg/µl
WB (with ECL): 1-10 ng of purified GFP or GFP fusion proteins
Store at -20°C or -80°C for one year. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.
PDF DatasheetMSDS
N/A
(Click image to enlarge)1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used
Wet ice shipping
N/A

Details

SpecificityRecognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFPIsotypeMouse IgG2bCloneGF28RPurificationProtein A affinity chromatography from mouse ascites fluidApplicationsDot, ELISA, IP, IS, WBWorking ConditionsWB (with ECL): 1:1000-3000 dilutionIS: 1:500-2000 dilutionFor best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration testImages(Click image to enlarge)1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used

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