This kit is designated for monitoring CHIP-mediated ubiquitination of user-supplied misfolded proteins. CHIP is an E3 ubiquitin ligase that directly or via Hsp70 interacts with misfolded proteins and promotes their polyubiquitination, which targets misfolded proteins for proteasomal degradation.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

Misfolded Protein Ubiquitination Kit

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J5110

1 Kit

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N/A

Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

See tube label

Enzyme >95% by SDS-PAGE

Store at -80°C; avoid multiple freeze-thaw cycles

PDF datasheet, MSDS

N/A

No

Dry ice shipping

N/A

Details

* This kit is enough for setting up 20 x 20 µl reactions.DescriptionThis kit is designated for monitoring CHIP-mediated ubiquitination of user-supplied misfolded proteins. CHIP is an E3 ubiquitin ligase that directly or via Hsp70 interacts with misfolded proteins and promotes their polyubiquitination, which targets misfolded proteins for proteasomal degradation. Components• 10x Human Ubiquitin (500 uM)..............................................40 µl• 20x UBE1 (E1, 2 uM))............................................................20 µl• 10x 6xHis-UbE2D3 (E2, 20 uM)............................................40 µl• 20x 6XHis-CHIP (E3, 40 uM))................................................20 µl• 10x Hsp70 (20 uM)................................................................40 µl• 5x Ubiquitination Buffer.......................................................150 µl• 10x ATP (40 mM)...................................................................60 µl

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