Ube2M is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for conjugation of the Ub-like protein NEDD8 to protein substrates. This E2 is unique from Ube2F in the fact that it does not need to complex with an E3 in order to conjugate the NEDD8 to a Cullin Ring Ligase (CRL), which is a subclass of Ub E3s. The major CRL for Ube2M is cullin-4A.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

6xHis-Ube2M (UbcH12)

UBC12; hUbc12; UBC-RS2

C3101

100 µg

20.9 kDa

Human

Bacterial recombinant

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

See tube label

~95% by SDS-PAGE, see datasheet for gel image

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Datasheet， MSDS

NM_003969

(Click image to enlarge)

Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified 6xHis-Ube2M (UbcH12)

Dry ice shipping

1. Osaka F, et al. (1998) Genes Dev 12(15), 2263 – 2268.2. Gong L, et al. (1999) J Biol Chem 274(17), 12036 – 12042.3. Huang DT, et al. (2009) Mol Cell 33, 483–495.4. Huang AM, et al. (2011) Biochemical Pharmocology 82, 35 – 42.

Details

Ube2M is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for conjugation of the Ub-like protein NEDD8 to protein substrates. This E2 is unique from Ube2F in the fact that it does not need to complex with an E3 in order to conjugate the NEDD8 to a Cullin Ring Ligase (CRL), which is a subclass of Ub E3s. The major CRL for Ube2M is cullin-4A. Images:(Click image to enlarge) Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified 6xHis-Ube2M (UbcH12)

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