Anti-β-Tubulin (BT7R) Loading Control Mouse Monoclonal Antibody

Additional Information

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Anti-beta-Tubulin

Anti-β-Tubulin (BT7R) Loading Control Mouse Monoclonal Antibody

Y1061

100 µg

~55/25 kDa

Human

Immunogen: β-Tubulin N-terminal peptide-KLH conjugates

10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)

1 µg/µl

Guaranteed for detecting endogenous β-Tubulin in 20 µg cell or tissue lysates

Store at -20°C or -80°C for one year. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.

PDF Datasheet， MSDS

N/A

(Click image to enlarge)Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)vFigure 2. 1:4000 (0.25 μg/ml) Ab dilution used in WB of 10 μg/lane (1), 5 μg/lane (2), 2.5 μg/lane (3) and 1.25 μg/lane (4) mouse brain tissue lysates

No

N/A

Details

SpecificityRecognizes native and denatured forms of β-Tubulin (~50kDa)IsotypeMouse IgG2aIsotypeMouse IgG2aCloneBT7RPurificationProtein A affinity chromatography from mouse ascites fluidApplicationsDot, ELISA, IS, WBCross Reactivityβ-Tubulin from human, monkey, mouse, rat, rabbit, chicken. β-Tubulin from other species may also be detectableWorking ConditionsWB (with ECL): 1:1000-5000 dilutionFor best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration testImages(Click image to enlarge)Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)vFigure 2. 1:4000 (0.25 μg/ml) Ab dilution used in WB of 10 μg/lane (1), 5 μg/lane (2), 2.5 μg/lane (3) and 1.25 μg/lane (4) mouse brain tissue lysates

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