The E2 Screening Kit is developed for identification of the E2 Ub-conjugating enzyme(s) for a specific E3 Ub ligase and/or a protein substrate. N/A

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

Customized E2 Screening Kit

N/A

J1200

1 Kit

N/A

Human

Bacterial recombinant

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

See tube label

90% - 95% based on SDS-PAGE

Store at -80°C; avoid multiple freeze-thaw cycles

PDF datasheet, MSDS

N/A

No

Dry ice shipping

N/A

Details

* When place an order, please select any ten (10) E2s from the list on the right side of this page.DescriptionThe E2 Screening Kit is developed for identification of the E2 Ub-conjugating enzyme(s) for a specific E3 Ub ligase and/or a protein substrate. Components• 20X UBE1 (2 µM).........................................................50µl• 10X E2 Ub conjugating enzymes (pick 10 E2s from the list below, 20µM)......................20 µl each• 10X Human Ubiquitin (500 µM).................................100 µl• 10X Ubiquitination Buffer...........................................500 µl• 40 mM ATP................................................................125 µlE2 Name1: 6XHis-Ube2A (Rad6A)2: 6XHis-Ube2B3: 6XHis-Ube2C(UbcH10 )4: 6XHis-Ube2D1 (UbcH5A)5: 6XHis-Ube2D2 (UbcH5B)6: 6XHis-Ube2D3 (UbcH5C)7: 6XHis-Ube2D4 (UbcH5D)8: 6XHis-Ube2E1 (UbcH6)9: 6XHis-Ube2E2 (UbcH8)10: 6XHis-Ube2E3 (UbcH9)11: 6XHis-Ube2F12: 6XHis-Ube2G113: 6XHis-Ube2G214: 6XHis-Ube2H (UbcH2)15: 6XHis-Ube2I16: 6XHis-Ube2J1_(1-282)17: GST-Ube2K (E2-25K)18: 6XHis-Ube2L319: 6XHis-Ube2L620: 6XHis-Ube2M (UbcH12)21: 6XHis-UbeN (Ubc13/Uev1a)22: 6XHis-Ube2Q223: 6XHis-Ube2R1 (Cdc34)24: 6XHis-Ube2R225: 6XHis-Ube2S26: 6XHis-Ube2T27: 6xHis-Ubc13/UbE2V228: 6XHis-Ube2W29: 6XHis-Ube2Z

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