Additional Information
Anti-HA |
Anti-HA (HA.C5) Mouse Monoclonal Antibody |
Y1070 |
25 µg |
~55/25 kDa |
N/A |
Immunogen: YPYDVPDYA (HA) synthetic peptide conjugated to KLH |
10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide) |
1 µg/µl |
Guaranteed for detecting 1-5 ng of HA-tagged fusion proteins |
Store at -20°C or -80°C for one year. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. |
PDF Datasheet, MSDS |
N/A |
(Click image to enlarge)1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (lane 1) and transfected (lane 2) |
Wet ice shipping |
N/A |
Details
SpecificityRecognizes the N-terminal, C-terminal or internal HA- tagged fusion proteinsIsotypeMouse IgG3CloneHA.C5PurificationProtein A affinity chromatography from mouse ascites fluidApplicationsDot. ELISA, IP, IS, WBWorking ConditionsWB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)IS: 1:500-2000 dilutionFor best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration testImages(Click image to enlarge)1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (lane 1) and transfected (lane 2)>>> 更多资讯详情请访问蚂蚁淘商城