Anti-β-Actin (BA3R) Loading Control Mouse Monoclonal Antibody

Additional Information

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Anti-beta-Actin

Anti-β-Actin (BA3R) Loading Control Mouse Monoclonal Antibody

Y1051

100 µg

~55/25 kDa

Human

Immunogen: β-Actin N-terminal peptide-KLH conjugates

10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)

1 µg/µl

Guaranteed for detecting endogenous β-Actin in 20 µg cell or tissue lysates

Store at -20°C or -80°C for one year. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.

PDF Datasheet， MSDS

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(Click image to enlarge)Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)Figure 2. 1:1000 (1 μg/ml) Ab dilution used in WB of 20 μg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4)

Wet ice shipping

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Details

SpecificityRecognizes native and denatured forms of β-Actin (~42kDa)IsotypeMouse IgG2b CloneBA3R PurificationProtein A affinity chromatography from mouse ascites fluidApplicationsDot, ELISA, IS, WBCross Reactivityβ-Actin from human, mouse, rat, rabbit, and chicken. β-Actin from other species may also be detectableWorking ConditionsWB (with ECL): 1:1000-5000 dilutionFor best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration testImages(Click image to enlarge)Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)Figure 2. 1:1000 (1 μg/ml) Ab dilution used in WB of 20 μg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4)

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