IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in _{E. coli}. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate. Note: To prepare 10 mL 1M IPTG stock solution, weight 2.38 g IPTG and dissolve in 8 ml distilled water, adjust to a final volume of 10 ml with distilled water. Sterile the solution using a 0.22 um syringe filter. Aliquot the stock and store under -20°C for up to one year.

Additional Information

Product Name: Also Known As: Catalog No.: Size: CAS: Formula: Molecular Weight: Form: Quality Assurance: Source: Storage: PDF Data Sheet: Image(s): Shipping Method: References:

IPTG

isopropyl - β - D - thiogalactopyranoside

P1010-500

5 X 100 g

367-93-1

C9H18O5S

238.3

Powder

>99% by HPLC, dioxane-free

Synthetic

Eligible for ambient temperature shipping. Store at 4°C upon receiving.

PDF Datasheet

Room temperature shipping

J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, A1.27, 2001) pp. 1.124-1.125.

Details

IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in _{E. coli}. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate. Note: To prepare 10 mL 1M IPTG stock solution, weight 2.38 g IPTG and dissolve in 8 ml distilled water, adjust to a final volume of 10 ml with distilled water. Sterile the solution using a 0.22 um syringe filter. Aliquot the stock and store under -20°C for up to one year. Expression of GST-UCHL3 in BL21 (DE3) cells was induced by the addition of 25 μM (lane 3), 100 μM (lane 4), 200 μM (lane 5) or 400 μM (lane 6) IPTG at 37 0C for 2 hours. Lane 2 without IPTG.

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