PS-341 is a specific, reversible and cell permeable dipeptide boronic acid inhibitor of the chymotrypsin-like activity of the proteasome (Ki=0.6nM). It inhibits proliferation of various tumor cell lines with nanomolar potency. Its commercial version was approved by the USFDA for the treatment of relapsed multiple myeloma. The typical concentrations for cell culture use are 1-20 µM. For in vitro use, the typical concentrations are 10-100 µM.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

PS-341

Bortezomib; VELCADE; PS341; MG-341; MG341; Pyz-Phe-boroLeu; CAS# 179324-69-7

F1202

100 mg

384.2 Da

N/A

Synthetic

Powder

N/A

>98% by HPLC and NMR

Eligible for room temperature shipping. Store at -80°C upon receiving; avoid multiple freeze-thaw cycles after dissolving in DMSO.

Download PDF datasheet

N/A

No

Room temperature shipping

1. Yi-He Ling et al. (2002) Mol Cancer Ther, 1:841-849. 2. Adams J. (2002) The Oncologist, 7:9-16. 3. Ling, Y., et al. (2003).Clin.Cancer Res.9,1145-1154.4. Chauhan, D., et al. (2005) Cancer Cell 8: 407-419.5. Dudek SA et al. (2010) J Virol. 84:9439-51.

Details

PS-341 is a specific, reversible and cell permeable dipeptide boronic acid inhibitor of the chymotrypsin-like activity of the proteasome (Ki=0.6nM). It inhibits proliferation of various tumor cell lines with nanomolar potency. Its commercial version was approved by the USFDA for the treatment of relapsed multiple myeloma. The typical concentrations for cell culture use are 1-20 µM. For in vitro use, the typical concentrations are 10-100 µM. Images(Click image to enlarge)100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 µM PS-341 for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 µM SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM PS-341 (lane 2)-treated HEK293T cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. Cells were treated with DMSO or PS-341 for 4 hours.

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