N-terminal TARMA-Ub (also called TARMA-Ub) is a fluorescent Ub, in which TAMRA (5-tetramethylrhodamine) is covalently conjugated on the N-terminus of Ub. All seven lysine residues in TARMA-Ub are available for ubiquitination. This product can be used for determination of protein ubiquitination or E1/E2/E3 enzyme activity using sensitive in-gel fluorescence. TAMRA fluorescence is detected using excitation/emission wavelengths at 550 nm/590 nm, respectively.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

N-terminal TAMRA-Ub

TAMRA-Ub

E1160

100 ug

8977 Da

Human

Chemical synthesis

20 mM Tris, pH 7.6 at 4 °C, 150 mM NaC and 10% glycerol

See tube label

>95% by FPLC.

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Datasheet， MSDS

N/A

See PDF datasheet.

Dry ice shipping

(1) El Oualid et al. Angew. Chem. Int. Ed. 2010, 49, 10149. (2) de Jong et al. ChemBioChem 2012, 13, 2251. (3) Smit et al. J Biol. Chem 2013, 288, 31728.

Details

N-terminal TAMRA-Ub (also called TAMRA-Ub) is a fluorescent Ub, in which TAMRA (5-tetramethylrhodamine) is covalently conjugated on the N-terminus of Ub. All seven lysine residues in TARMA-Ub are available for ubiquitination. This product can be used for determination of protein ubiquitination or E1/E2/E3 enzyme activity using sensitive in-gel TAMRA fluorescence. TAMRA fluorescence is detected using excitation/emission wavelengths at 550 nm/590 nm, respectively. See images in PDF datasheet.

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