InhibitionofPARP1by3-aminobenzamide
<clicktoimageenlarge>
<clicktoenlarge>
AutomodificationofPARP1WBusinganti-PARP1
<clicktoenlarge>
●PARP1Enzymehuman, highlyactivePoly(ADP-ribose)polymerase-1(PARP1)isanabundantandubiquitousnuclearenzymethatcatalyzestheNAD(+)-dependentadditionofADP-ribosepolymersonavarietyofnuclearproteins. TheTulipBiolabs,Inc. PARP1Enzyme,Cat.#2090,ishighlypurifiedhumanPARP1,expressedinabaculovirusexpressionsystem.ThePARP1hasahighspecificactivity,approx.20000U/mg(1U=10fmolADP-riboseincorporatedintoimmobilizedhistonein30min@RT). Itislyophilizedfromitsnativestateunderspecialconditions,anduponreconstitutionwithwater,itretainsitspolyADP-ribosylationenzymaticactivity. PuritybySDS-PAGEis>95%. Eachvialcontains50µgPARP1(approx.1000Units).
●Useinhigh-throughputenzymeactivity/inhibitionassays
●Poly-ADP-ribosylationoftargetproteins
●WesternBlot,100ng/laneusingHRP/TMBcolorimetricmethods
●ELISA,2ng/well
●SDS-PAGE,1µg/lane●Asastandardinotherassays
Pleaseseethe Cat.#2090Datasheet(pdf) foracompletedescription.
