Description
ThehighcellularityofcardiactissuepresentsproblemsinpermeABIlization,sotheCardioTACS™Kitcomeswithtwopermeabilizationreagentstoprovideoptions.ThekitisbasedonDNAend-labelingusingterminaldeoxynucleotidyltransferase(TdT)andamodifiednucleotidethatissubsequentlydetectedusingourTACSBlueLabel™detectionsystem.TrevigenhasdevelopedanexclusiveapoptosisgradeTdTenzymethatwhencoupledwiththeTACSBlueLabel™solution,provideslabeling20to50timesmoresensitivethanstandarddiaminobenzidine(DAB)labelingmethods.Toensureeaseofdatainterpretationwhenthenumbersofapoptoticcellsarelow,CardioTACS™includesNuclearFastRedCounterstainwhichprovidessuperbcontrasttotheTACSBlueLabel™inapoptoticcells.
Inaddition,thekitincludesTACS-Nuclease™togenerateapositivecontrolinyourownsample.Thepositivecontrolprovidesaninternalcontrolforpermeabilizationandlabelingsoadditionaloptimizationisnominalandthedatacanbeinterpretedwithconfidence.Thiscompletekitprovidesallthereagentsrequiredforlabelingincludingtwopermeabilizationreagents,labelinganddetectionreagents,stopbuffer,counterstain,TACS-Nuclease™reagent,andaprotocoldetailingincubationtimesandreagentconcentrations.
FEATURES:
- Performancetestedonheart-derivedsamples.
- IncludesbothProteinaseKandexclusiveCytonin™non-enzymaticpermeabilizationreagents.
- IncludesTACS-Nuclease™solutionforpreparingsample-dependentpositivecontrols.
- Helpsresolveuniqueproblemsencounteredwhendetectingapoptoticcardiaccells.
APPLICATIONS:
- Insitudetectionofapoptosisinfixedfrozen,paraffinembedded,orplasticembeddedcardiaccellsandtissues.
- Assistsintheidentificationofapoptoticmorphologies.
| Catalog#4827-30-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 4800-30-01 | ProteinaseK | 1 |
| 4800-30-06 | Streptavidin-HRP | 1 |
| 4800-30-11 | TACS-BlueLabel™ | 1 |
| 4800-30-12 | BlueStrep-HRPDiluent | 1 |
| 4800-30-15 | TACS-Nuclease™ | 1 |
| 4800-30-16 | TACS-Nuclease™Buffer | 1 |
| 4800-30-17 | NuclearFastRed | 1 |
| 4810-30-02 | TACS®2TdTLabelingBuffer | 1 |
| 4810-30-03 | TACS®2TdTStopBuffer | 1 |
| 4810-30-04 | TACS®2TdTdNTP | 1 |
| 4810-30-05 | TdTEnzyme | 1 |
| 4810-30-14 | 50xManganeseCation | 1 |
| 4876-05-01 | Cytonin™ | 1 |
TheInfluenceofAgeonSerumConcentrationsofCardiacTroponinI:ResultsinRats,Monkeys,andCommercialSera
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“OleksandraPrysyazhna,JosephRobertBurgoyne,JennaScotcher,StevenGrover,DavidKass,andPhilipEaton”
J.Biol.Chem.,Aug2016;291:17427–17436.
http://www.jbc.org/cgi/content/abstract/291/33/17427
