Description
CONCENTRATION
500unitspervial
SOURCE
PurifiedfromE. colicontainingarecombinantplasmidharboringtheMethanobacteriumThermoautotrophicumTDGgene.
SUBSTRATESPECIFICITY
RecognizesT/GmismatchesinduplexDNAandcleavesthestrandwiththeT.Also,recognizesG/GmismatchesifatleastonenearestneighborisanAorTandnicksonestrandortheother.
STORAGE
Storeat-20°Cinamanualdefrostfreezer.Forlong-termstorage,freezeat-80°Cinworkingaliquots.
APPLICATIONS
DNARepairAssays
Catalog#4070-500-EBincludes: | ||
CatalogNumber | Description | Qty |
3900-500-04 | 10XREC™ReactionBuffer4 | 1 |
4070-500-01 | ThermostableTDGEnzyme | 1 |
DNAcytosineandmethylcytosinedeaminationbyAPOBEC3B:enhancingmethylcytosinedeaminationbyengineeringAPOBEC3B
YangFu,FumiakiIto,GewenZhang,BraulioFernandez,HanjingYang,andXiaojiangS.Chen
Biochem.J.,Oct2015;471:25–35.
http://www.biochemj.org/cgi/content/abstract/471/1/25