Description
4418-096-k_calculation_worksheet
InanELISAassay,immobilizedgamma-H2AXantibodyinthewellsofa96-wellplatecapturesgamma-H2AXfromsamplelysate. IncubationwithaH2AXdetectingantibody,followedbyadditionofagoatanti-mouseHRPconjugateandachemiluminescentHRPsubstrateyieldsrelativelightunits(RLU)thatdirectlycorrelateswiththeamountofgamma-H2AXinthesample.Thegamma-H2AXpolyclonalantibodymaybepurchasedseparately.
FEATURES:
- Chemiluminescent,non-rADIoactiveELISA
- Pre-coated96wellcaptureantibodyplate
- Dynamicrangefrom10pMto800pM
- Sensitivitywith5pMofgamma-H2AX
APPLICATIONS:
Quantitationofgamma-H2AXlevelsinperipheralbloodmononuclearcells,tissueculturecells,andtissuebiopsies.
| Catalog#4418-096-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 4418-096-01 | Gamma-H2AXStandard,1µM | 1 |
| 4418-096-06 | JurkatCellLysateControl | 1 |
| 4418-096-02 | AssayBuffer | 1 |
| 4418-096-05 | CellLysisReagent | 1 |
| 4418-096-07 | 25XWashBuffer | 1 |
| 4418-096-P | 96-Stripwellpre-coatedplatewith3filmsealers | 1 |
| 4675-096-01 | PARPPeroxyGlow™A | 1 |
| 4675-096-02 | PARPPeroxyGlow™B | 1 |
| 4418-096-03 | H2AXIgMDetectingAntibody | 1 |
| 4418-096-04 | Goatanti-MouseIgMHRPConjugate | 1 |
Abstract2423:DevelopmentofavalidatedhighthroughputELISAassayforgammaH2AXasapharmacodynamicMarker
JayGeorgeandHaiXu
CancerRes.,Oct2014;74:2423.
http://cancerres.aacrjournals.org/cgi/content/abstract/74/19_Supplement/2423}
Engineeredcellulargene-replacementplatformforselectiveandinducIBLeproteolyticprofiling
CharlesW.Morgan,JuanE.Diaz,SamanthaG.Zeitlin,DanielC.Gray,andJamesA.Wells
PNAS,Jul2015;112:8344–8349.
http://www.pnas.org/cgi/content/abstract/112/27/8344
