Description

7512-100-k_cuvette_assay_calculation_worksheet

7512-100-k_plate_assay_calculation_worksheet

TetramericGlutathionePeroxidasecatalyzesthereductionofH2O2towater,andorganicperoxidestothecorrespondingstablealcohols,byusingglutathioneasasourceofreducingequivalents.Itrequiresseleniumasacofactorandcontainsaselenocysteineaminoacidresidueintheactivesiteofeachmonomerthatparticipatesintheactualmechanismoftheenzyme.GlutathioneReductase(GR),providedwitheachkit,thenreducestheoxidizedglutathionetocompletethecycle.TheoxidationofNADPHtoNADP+isaccompaniedbyadecreaseinabsorbanceat340nm.Therateofdecreaseintheabsorbanceat340nmisdirectlyproportionaltotheGlutathionePeroxidaseactivityinthesample.

FEATURES:

• Suitablefora96wellorcuvetteformat
• Suitableforplasma,erythrocytelysates,tissuehomogenates,andcelllysates

APPLICATIONS:

Calculatingglutathioneperoxidaseactivity.

 

Catalog#7512-100-Kincludes:
CatalogNumber	Description	Qty
7512-100-01	GlutathionePeroxidase	1
7512-100-02	10XAssayBuffer	1
7512-100-03	GSH+NADPH	1
7512-100-04	GlutathioneReductase	1
7512-100-05	CumeneHydroperoxide	1
7512-100-06	96-wellplates	1

Glutathione-MediatedDetoxificationofHalobenzoquinoneDrinkingWaterDisinfectionByproductsinT24Cells
JinhuaLi,WeiWang,HongquanZhang,X.ChrisLe,andXing-FangLi
Toxicol.Sci.,Oct2014;141:335–343.
http://toxsci.oxfordjournals.org/cgi/content/abstract/141/2/335

Trevigen公司位于美国马里兰州的高科技走廊，是一家有12年历史、成长迅速的生物技术公司，其产品和技术的发展方向主要在癌症研究领域，重点强调细胞凋亡、DNA损伤和修复，以及癌症细胞功能和行为。Trevigen品牌的分销商遍及北美，欧洲和环太平洋地区。关于DNA的损伤分析技术的研究，Trevigen获得美国国立卫生研究院（NIH）的SBIR项目资助。