Description

IntheSuperoxideDismutaseAssay,ionsgeneratedfromtheconversionofxanthinetouricacid,andhydrogenperoxidebyxanthineoxidase(XOD),convertNBTtoNBT-diformazan.NBT-diformazanabsorbslightat550nm.SODsreducesuperoxideionconcentrationsandtherebylowertherateofNBT-diformazanformation.TheextentofreductionintheappearanceofNBT-diformazanisameasureofSODactivitypresentinexperimentalsamples.Theassayisfreeofinterferencebyothercatalyticactivities,andisidealforassayingSODinmammaliancelllysates.Thekitcontainstheproperlysisbufferandthereagentsneededfor100experimentaltests,50positivecontrols,and50negativecontrols.UnlikesomeotherassaykitsforSOD,thissystemisnotgreatlydisturbedbytracemetals.Eachassayrequiresonlyaboutfiveminutes,andafterasimplecalculation,thepercentinhibitionoftheformationofNBT-diformazanbySODisconvertedtotherelativeactivityofthesample.

FEATURES:

• Suitableformammaliancells.
• Eachsampletakesonly5minutes.
• ContainsSODfor50positivecontrols.
• Suitablefortheassayof(Mn²⁺)-SOD,(Fe²⁺)-SOD,and(Cu/Zn)^–SOD.

APPLICATIONS:

CalculatingSODactivitybymeasuringthereductionofNBT-diformazanincellextracts.

 

Catalog#7500-100-Kincludes:
CatalogNumber	Description	Qty
7500-100-01	SuperOxideDismutase	1
7500-100-05	XODSolution	1
7500-100-02	25XSODReactionBuffer	1
7500-100-03	XanthineSolution	1
7500-100-04	NBTSolution	1
7500-100-06	20XCellLysisSolution	1

RosiglitazoneCausesCardiotoxicityviaPeroxisomeProliferator-ActivatedReceptor{gamma}-IndependentMitochondrialOxidativeStressinMouseHearts
HuameiHe,HaiTao,HuiXiong,ShengZhongDuan,FrancisX.McGowan,Jr.,RichardM.Mortensen,andJamesA.Balschi
Toxicol.Sci.,Apr2014;138:468–481.
http://toxsci.oxfordjournals.org/cgi/content/abstract/138/2/468

Protectiveactivityofanovelresveratrolanalogue,HS-1793,againstDNAdamagein137Cs-irrADIatedCHO-K1cells
Jeong,M.H.,Yang,K.M.,Jeong,D.H.,Lee,C.G.,Oh,S.J.,Jeong,S.K.,Lee,K.W.,Jo,Y.R.andJo,W.S.
J.RadiatRes,May2014;55:464–47
http://jrr.oxfordjournals.org/cgi/content/abstract/55/3/464

Trevigen公司位于美国马里兰州的高科技走廊，是一家有12年历史、成长迅速的生物技术公司，其产品和技术的发展方向主要在癌症研究领域，重点强调细胞凋亡、DNA损伤和修复，以及癌症细胞功能和行为。Trevigen品牌的分销商遍及北美，欧洲和环太平洋地区。关于DNA的损伤分析技术的研究，Trevigen获得美国国立卫生研究院（NIH）的SBIR项目资助。