Description
7501-500-k_calculation_worksheet
IntheHTSuperoxideDismutaseAssay,superoxiderADIcalions,generatedfromtheconversionofxanthinetouricacid,andhydrogenperoxidebyxanthineoxidase(XOD),convertWST-1toWST-1-formazan.WST-1-formazanabsorbslightat450nm.SODsreducesuperoxideionconcentrationsandtherebylowertherateofWST-1formazanformation.TheextentofreductionintheappearanceofWST-1formazanisameasureofSODactivitypresentinexperimentalsamples.Thisassayisfreeofinterferencebyothercatalyticactivities,andunlikesomeotherassaykitsforSOD,thissystemisnotgreatlydisturbedbytracemetals.
FEATURES:
- 96wellformatforhighthroughputscreening
- Assayperformedinaslittleas6minutes
- Suitableformammaliantissuesandcelllysates
- Suitablefortheassayof(Mn2+)-SOD,(Fe2+)-SOD,and(Cu/Zn)-SOD
APPLICATIONS:
CalculatingrelativeSODactivitybymeasuringthepercentinhibitionoftherateofformationofWST-1formazan.
Catalog#7501-500-Kincludes: | ||
CatalogNumber | Description | Qty |
7501-500-01 | SODStandard | 1 |
7501-500-03 | XanthineOxidase(XOD) | 1 |
7501-500-02 | 10XSODBuffer | 1 |
7501-500-04 | 10XXanthineSolution | 1 |
7501-500-05 | 20%TritonX-100 | 1 |
7501-500-06 | WST-1Reagent | 1 |
7512-100-06 | 96-WellPlate | 1 |
Trevigen公司位于美国马里兰州的高科技走廊,是一家有12年历史、成长迅速的生物技术公司,其产品和技术的发展方向主要在癌症研究领域,重点强调细胞凋亡、DNA损伤和修复,以及癌症细胞功能和行为。Trevigen品牌的分销商遍及北美,欧洲和环太平洋地区。关于DNA的损伤分析技术的研究,Trevigen获得美国国立卫生研究院(NIH)的SBIR项目资助。