Description
Cultrex®StemCellQualifiedHuman Vitronectin,Pathclear®ispurifiedfromhumanplasmaandisPathclear®certifiedtobefreeofcommonpathogens.Vitronectinisanextracellular,soluble,disulfide-linkeddimer,composedofa75kDaanda65kDapeptidechainwithatotalmolecularweightof140kDa.VitronectinisamajorplasmaglycoproteinthatpromotescellularadhesionandspreADIng.Italsoinhibitsthemembranedamagingeffectoftheterminalcytolyticcomplementpathwayandbindstoseveralserpinserineproteaseinhibitors.Vitronectin,alongwithcollagenIV,fibronectin,andlaminincansupportrobust,longtermproliferationofundifferentiatedhumanembryonicstemcells.Vitronectincanbeusedforcoatingtissueculturesurfacestopromotecelladhesion,proliferationanddifferentiation,orasanadditiveforserum-freemedia.Vitronectinispurifiedfromhumanplasmaandis0.2μmsterilefiltered.
Cultrex®StemCellQualifiedHumanVitronectin,Pathclear®isapurifiedplasmaextracellularmatrix proteinthathasbeendeveloped,producedandqualifiedtoprovideafunctionallydefinedandeffectivefeeder-freesurfacefortheattachmentandmaintenanceofembryonicstemcellsinapluripotentstate.
Qualitycontrolspecifications:
- PathClear®–testednegativeforeighthumanpathogenicvirusesincludingHepatitisA,BandC,HIV1and2,Hantaan,Seoul,andSinNombrebyPCR.
- Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
- Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
- Proteinconcentrationof1mg/ml.
- NomycoplasmacontaminationdetectedbyPCR.
- Providedin10mMSodiumPhosphate,pH7.7,8MUrea,5mMEDTA,500mMNaCl.
- Purity ≥90%(SDS-PAGE).
- BIOLOGicalactivitydeterminedforeachlot:
- PromotestheattachmentofhumaniPSCs.
- EffectivelymaintainshumaniPSCsinapluripotentstateinafeeder-freeculture.
SafetyStatement:
AlthoughCultrex®StemCellQualifiedHumanVitronectin,PathClear®hasbeentestedforthevirusesaboveitcontainshumansourcematerialandthereforeshouldbetreatedaspotentiallyinfectiousandhandledattheBiologicalSafetyLevel2tominimizeexposure.
Trevigen公司位于美国马里兰州的高科技走廊，是一家有12年历史、成长迅速的生物技术公司，其产品和技术的发展方向主要在癌症研究领域，重点强调细胞凋亡、DNA损伤和修复，以及癌症细胞功能和行为。Trevigen品牌的分销商遍及北美，欧洲和环太平洋地区。关于DNA的损伤分析技术的研究，Trevigen获得美国国立卫生研究院（NIH）的SBIR项目资助。
