Description

NOTE: StartingJanuary1st,2017freesamplesizesof Cultrex® OrganoidHarvestingSolution, Cultrex®BMEType2, and Cultrex®BMETypeR1 willbeaddedtoyourorder. Theseproductsarealsosoldindividually,moreinformationonTrevigen®’sCultrexOrganoidproductscanbefoundhere.

Roofplate-specificSpondin-1(R-Spondin1orRspo1),alsoknownasCRISTIN3,isa27kDasecretedactivatorproteinthatbelongstotheR-Spondinfamily.R-SpondinspositivelyregulateWnt/beta-cateninsignaling,mostlikelybyactingasaligandforLGR4-6receptorsandaninhibitorforZNRF3.R-Spondin-1inducesproliferationofintestinalcryptepithelialcells,increasesintestinalepithelialhealing,andsupportsintestinalepithelialstemcellrenewal.

Features:

• RobustandstableexpressionofR-Spondin1.
• Twoepitopetagsprovideflexibilitytopurifyanddetecttheexpressedprotein.
• AccesIBLesourceofeitherpurifiedR-Spondin1orR-Spondin1conditionedmedium.
• Suitabletosupplementbothhumanandmurineorganoidculturemedium.

Qualitycontrolspecifications:

• NegativebyPCRforMycoplasma.
• ExpressiondetectedbyWesternBlot–Abandofapproximately70kDacorrespondingtoHA-R-Spondin1-Fcisdetectedintheconditionedmediumofcellsafter7daysofculture.
• BIOLOGicalactivitydeterminedforeachlot:
  • TopFlashassay–HA-R-Spondin1-FcmustinducetheexpressionofluciferaseunderthecontrolofaTCF/LEFpromoter.

Trevigen公司位于美国马里兰州的高科技走廊，是一家有12年历史、成长迅速的生物技术公司，其产品和技术的发展方向主要在癌症研究领域，重点强调细胞凋亡、DNA损伤和修复，以及癌症细胞功能和行为。Trevigen品牌的分销商遍及北美，欧洲和环太平洋地区。关于DNA的损伤分析技术的研究，Trevigen获得美国国立卫生研究院（NIH）的SBIR项目资助。