Description
Organoidculturesexhibitcellularbehaviorsandmorphologiessimilartothoseseeninvivo,however,theadaptationofthesemodelsforstudyingbiochemicalprocesseshasbeenimpededbythechallengeofseparatingintactorganoidsfromextra-cellularproteinscomprisingthehydrogel.Commonly,proteasesareemployedtodegradetheseextracellularproteins,however,proteasesalsodegradeproteinsonthecellsurfaceandproteaseactivitymaycarryoverintosubsequentculturesorlysatepreparations.Trevigen’sCultrex®OrganoidHarvestingSolutionprovidesanon-enzymaticmethodfordepolymerizingextracellularmatrixproteinstoallowforharvestingofintactorganoidsforpassaging,cryopreservation,orbiochemicalanalysis.
Qualitycontrolspecifications:
Functionalassay–TenvolumesofOrganoidHarvestingSolution(500μl)depolymerizesa50μldomeofbasementmembranematrixina24wellplatewithmoderateshakingonicefor90minutes.
Sterilitytesting–Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14days.
Endotoxinconcentration≤20EU/mlbyLALassay.
StorageConditions:
Productisstableforatleast6monthsfromthedateofreceiptwhenstoredat2–8°C.Keepsterile.
Trevigen公司位于美国马里兰州的高科技走廊，是一家有12年历史、成长迅速的生物技术公司，其产品和技术的发展方向主要在癌症研究领域，重点强调细胞凋亡、DNA损伤和修复，以及癌症细胞功能和行为。Trevigen品牌的分销商遍及北美，欧洲和环太平洋地区。关于DNA的损伤分析技术的研究，Trevigen获得美国国立卫生研究院（NIH）的SBIR项目资助。
