TheNeu5,9Ac2standardstandardisamixtureofvarioussialicacids.Themainsialicacidspeciespresent,5-N-acetyl-9-O-acetylneuraminicacid(Neu5,9Ac2),makesup77%ofthemixture.Thisproductisonlyforuseasachromatographystandardandshouldbenotusedasaquantitativestandard.
LudgerGuidestoSialicAcidAnalysis:
SialylationGuideI:Neu5AcandNeu5GcQuantitation
SialylationGuideII:HighlySialylatedGlycoproteins
ApplicationNote:QuantitativeSialicAcidAnalysis
LudgerSep-R1HPLCprofileof1,2-diamino-4,5-methylenedioxybenzene.2HCl(DMB)labeledNeu5,9Ac2standard
Form:Dry.Driedbycentrifugalevaporationfromanaqueoussolution.
AmountSupplied:~1nmol(ofwhich770pmolisNeu5,9Ac2)
Storage: -20˚Cbothbeforeandafterdissolution.Thisproductisstableforatleast5yearsassupplied.
ViewCertificateofStABIlty
Shipping:TheNGNAstandardcanbeshippedatambientwhendry.Afterdissolution,shipondryice.
Handling:Allowtheunopenedvialtoreachambienttemperatureandtapunopenedonasolidsurfacetoensurethatmostofthelyophilizedmaterialisatthebottomofthevial.Gentlyremovethecap,addthedesiredvolumeofreconstitutionmedium,re-capandmixthoroughlytobringalltheoligosaccharideintosolution.Formaximalrecoveryofoligosaccharide,ensurethatthecapliningisalsorinsedandcentrifugethereconstitutedvialbrieflybeforeuse.Ensurethatanyglass,plasticwareorsolventsusedarefreeofglycosidasesandenvironmentalcarbohydrates.MinimiseexposuretoelevatedtemperaturesorextremesofpH.HightemperaturesandlowpHwillcausedesialylation.HighpHwillcauseepimerisationofthereducingterminusGlcNAc.
Safety:Thisproductisnon-hazardousandhasbeenpurifiedfromnaturalsourcescertifiedtobefreeofallhazardousmaterialincludingpathogenicBIOLOGicalagents.
DownloadNon-hazardousMSDS
References:
Fernandes,D.BiopharmaceuticalSialylation.EuropeanBiopharmaceuticalReview.Spring2006.pp100-104
BhideGP,ColleyKJ.SialylationofN-glycans:mechanism,cellularcompartmentalizationandfunction.HistochemCellBiol.2017Feb;147(2):149-174.doi:10.1007/s00418-016-1520-x.Epub2016Dec14.
AnumulaKR.Rapidquantitativedeterminationofsialicacidsinglycoproteinsbyhigh-performanceliquidchromatographywithasensitivefluorescencedetection.AnalyticalBiochemistry230:pp24-30,1995,
QA-bio公司是一家致力于提供糖基化研究工具的专业公司，所提供的糖基化研究产品涵盖糖苷内/外切酶，去糖基化酶，唾液酸产品，多糖产品纯化试剂盒，多糖标记试剂盒，HPLC柱及其缓冲液，同时公司还提供多糖分析服务。此外，公司还提供部分分子生物学产品。
