品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com

QA-Bio/2-AA Labeling Kit/LT-KAA-VP24

价格
¥6722.82
货号:LT-KAA-VP24
浏览量:127
品牌:QA-Bio
服务
全国联保
正品保证
正规发票
签订合同
商品描述

The2-AAlabelingkitcontainstwosetsofthefollowingreagents(15samplesperkit)suppliedinglassampoulessealedunderpurenitrogen:  2-aminobenzoicacid(2-AAdye)  Dimethylsulfoxide(DMSO)  Aceticacid  Sodiumcyanoborohydrideor2-picolineborane

TrADItional2-ABand2-AAlabellingkitsusesodiumcyanoborohydrideasareducingagentduringglycanlabeling.Thisreagentistoxicsoafumecupboardshouldbeusedduringhandling.ToconformwithemerginghealthandsafetyregulationswearenowreplacingthesewithournewVPglycankitsthatusepicolineboranewhichisasignificantlysaferreductant.

NumberofSamplesOne2-AAlabelingkitcontainsreagentstolabelupto30separateanalyticalsamplesperkit.

Dyepurity>99%byHPLC

Molecularweight137

LamBDa-ex320nm

Lambda-em420nm

AmountofSampleFrom25pmolupto25nmolglycanspersample.

SuitableSamplesAnypurifiedglycanswithfreereducingterminicanbelabeled.

StructuralIntegrityNodetectable(<2molepercent)lossofsialicacid,fucose,sulfate,orphosphate.

LabelingEfficiencyTypically>85%(dependentonsample).

LabelingSelectivityEssentiallystoichiometriclabeling.

Protocol1. PurifytheglycansLudgerCleanEB10cartidges(LC-EB10-A6)havebeendesignedforpurificationofglycansfromproteins,salts,anddetergents.

2.TransfersampletoreactionvialTheamountofsampleshouldbeintherange100picomoles–50nanomolesforaglycanpoolobtainedfromatypicalglycoprotein.Withasinglepureglycanaslittleas5picomolescanbelabeledanddetectedinsubsequentHPLCanalysis.SuitablereactionvialsincludesmallpolypropylenemicrocentrifugetubesandtubesforPCRwork.

3. DrythesamplesIdeally,samplesshouldbedriedusingacentrifugalevaporator.IfthisisnotpossIBLethenfreezedrying(lyophilization)canbeusedwithcaution(inparticular,ensurethatthesampledriestoasmall,compactmassattheverybottomofthevial).Donotsubjectsamplestohightemperatures(>28°C)orextremesofpHastheseconditionswillresultinacidcatalysedlossofsialicacids(hightemperatures,lowpH)orepimerizationoftheglycanreducingterminus(athighpH).

4.PrepareaDMSO-aceticacidmixtureAdd150μLglacialaceticacidtothevialofDMSOandmixbyPipetteaction.

5.AddthedyeAdd100μLoftheDMSO-aceticacidmixturetoavialofthe2-AA(2-aminobenzoicacid)dyeandmixuntilthedyeisdissolved.

6.AddthereductantAddthedissolveddyetoavialofsodiumcyanoborohydrideorpicolineboraneandmixbypipetteactionuntilthereductantiscompletelydissolvedtomakethefinallabelingreagent.

7.Add2-AAlabelingreagenttosamplesAdd5μLoflabelingreagenttoeachdriedglycansample,capthemicrotube,mixthoroughly.

8.IncubatePlacethereactionvialsinaheatingblock,sandtray,ordryovensetat65°Candincubatefor3hours.Inmostcases,theincubationtimecanbeshortenedto2hoursorextendedupto4hourswithoutsignificantlychangingtheoutcomeofthelabelingreaction.

9.CentrifugeandcoolAftertheincubationperiodremovethesamples,centrifugethemicrotubesbriefly,thenallowthemtocoolcompletelytoroomtemperature.

10.SampleCleanupPost-labelingsamplecleanupisrecommendedtoremoveexcessdyeandotherlabelingreagents.CleanupcanbeachievedusingLudgerCleanT1cartridges(LC-T1-A6)orScartridges(LC-S-A6)

2-AALabelingReferences

Anumula,K.R.Quantitativedeterminationofmonosaccharidesinglycoproteinsbyhigh-performanceliquidchromatographywithhighlysensitivefluorescencedetection.AnalyticalBiochemistry220:275-283(1994)

Anumula,KR;Dhume,STHighresolutionandhighsensitivitymethodsforoligosaccharidemappingandcharacterizationbynormalphasehighperformanceliquidchromatographyfollowingderivatizationwithhighlyfluorescentanthranilicacid.GlycoBIOLOGy8:685-694(1998)

Anumula,KR;Du,PCharacterizationofcarbohydratesusinghighlyfluorescent2-aminobenzoicacidtagfollowinggelelectrophoresisofglycoproteins.AnalyticalBiochemistry275:236-242(1999)

Bigge,JC;Patel,TP;Bruce,JA;Goulding,PN;Charles,SM;Parekh,RBNonselectiveandefficientfluorescentlabelingofglycansusing2-aminobenzamideandanthranilicacid.AnalyticalBiochemistry230:229-238(1995)

Frears,ER;Merry,AH;Axford,JSScreeningneutralandacidicIgGN-glycansbyhighdensityelectrophoresis.GlycoconjugateJournal16:283-290(1999)

Huang,Z;Prickett,T;Potts,M;Helm,RFTheuseofthe2-aminobenzoicacidtagforoligosaccharidegelelectrophoresis.CarbohydrateResearch328:77-83(2000)

Sato,K;Sato,K;Okubo,A;Yamazaki,SDeterminationofmonosaccharidesderivatizedwith2-aminobenzoicacidbycapillaryelectrophoresis.strong>AnalyticalBiochemistry251:119-121(1997)

QA-bio公司是一家致力于提供糖基化研究工具的专业公司,所提供的糖基化研究产品涵盖糖苷内/外切酶,去糖基化酶,唾液酸产品,多糖产品纯化试剂盒,多糖标记试剂盒,HPLC柱及其缓冲液,同时公司还提供多糖分析服务。此外,公司还提供部分分子生物学产品。
  • 资质认证

    获得国家资质,权威认证!

  • 全国联保

    全国联保,官方无忧售后

  • 正规发票

    正规发票,放心购买

  • 签订合同

    签订合同,保障您的权益

/**/