ImmobilizedPepsinresin(PepsinAgarose)iscommonlyusedtocreateF(ab’)2fragmentsfromantibodies.Byimmobilizingpepsinautolysisandproteasecontaminationisessentiallyeliminated.ImmobilizedPepsingelhydrolyzesproteinnon-specificallyatpH1.0to3.0,withanoptimumactivityatpH1.0.Ourhighlyactivepepsiniscovalentlyboundtocrosslinked6%beadedagaroseresinanditsspecificactivityis>6,100units/mlresin(oneunitisdefinedasreleasing0.001A280asTCAhydrolyzed/min.at37°C).ProteoChem"sImmobilizedPepsinispackagedasaslurryinbufferatpH4.5containing50%glycerol,100mMSodiumAcetate,0.05%NaN₃.

Pepsinproteasecleavesnon-specificallywithsomepreferencefortyrosinesandphenylalanine(aromaticresidues),althoughitgenerallydoesnotcleaveatalanine,glycineorvalineresidues.RecommendedconditionsarepH1-3,20°Cto37°C. 净化洗涤剂-净化洗涤剂解决方案ProteoChem的纯化洗涤剂水溶液具有超高纯度，低过氧化物含量和低羰基含量。纯化的聚山梨酯20，聚山梨酯80，聚乙二醇十二烷基醚，NP-40，Triton X-100和Triton X-114的10％（w / v）溶液可用于增溶膜蛋白，减少非特异性结合ELISA或IHC，有助于细胞膜裂解和其他蛋白质研究应用。纯化洗涤剂溶液的药物用途包括稳定乳液和悬浮液。ProteoChem的纯化表面活性剂溶液是最好的。它们的稳定性和纯度处于行业领先地位（<1.0 µeq / ml的过氧化物和羰基化合物）。