NHS-SS-Biotin, Succinimidyl-2-(biotinamido)-ethyl-1,3"-dithiopropionate, is an amine-reactive biotinylation reagent that is cleavable when the disulfide (-S-S-) bond is reduced.NHS-SS-Biotin is cell membrane permeable and has a long chain separating the biotin group from the amino-reactive NHS-ester (N-Hydroxysuccinimide ester).The resulting biotinylated protein can then be detected when used in conjunction with streptavidin.It is possible to cleave the biotin group from the crosslinked target protein under reducing conditions, such as in the presence of beta-mercaptoethanol (BME), TCEP-HCl or DTT. This technique is often used to purify an antibody or other protein from a biotin specific affinity resin like Immobilized Streptavidin without exposing your protein of interest to denaturing conditions.

Click here for: NHS-SS-Biotin Protocol& Product Information Sheet

Molecular Weight: 504.65Spacer Length: 24.3 ÅCAS Number: 142439-92-7Alternate Names: Biotin disulfide N-Hydroxysuccinimide ester; NHS SS BiotinSpecial bulk pricing is available on bulk quantities of NHS-SS-Biotin for your large volume manufacturing application.

净化洗涤剂-净化洗涤剂解决方案ProteoChem的纯化洗涤剂水溶液具有超高纯度，低过氧化物含量和低羰基含量。纯化的聚山梨酯20，聚山梨酯80，聚乙二醇十二烷基醚，NP-40，Triton X-100和Triton X-114的10％（w / v）溶液可用于增溶膜蛋白，减少非特异性结合ELISA或IHC，有助于细胞膜裂解和其他蛋白质研究应用。纯化洗涤剂溶液的药物用途包括稳定乳液和悬浮液。ProteoChem的纯化表面活性剂溶液是最好的。它们的稳定性和纯度处于行业领先地位（<1.0 µeq / ml的过氧化物和羰基化合物）。