- 盐酸羟胺
- UltraPure MALDI Matrices
- 羧基反应树脂(固定化DADPA)
- Protein Crosslinkers & Protein Crosslinking Reagents
- 质量标准分析用双(磺基琥珀酸)2,2,4,4-戊二酸-d4
- 对硝基苯磷酸盐(pNPP)
- 维生素H
- 芥子酸(SA)MALDI-MS基质
- Biotinylation Reagents
- 2’、4’,-二羟基苯乙酮(DHAP)MALDI-MS基质
- 硼酸钠缓冲液
- 双(磺基琥珀酰亚胺基)2,2,7,7-亚硫酸盐-d4用于质谱分析
- 零长度交联剂-EDC-HCl,Sulfo-NHS和NHS
- 琥珀酰亚胺6-(生物胺基)己酸盐(NHS LC生物素)
- 三(2-羧乙基)膦盐酸盐(TCEP-HCl)
- 二硫双歧杆菌(琥珀酰亚胺-2,2,3,3,6,6,7,7-丙酸-d8)氘化交联剂
- 二甲基甲酰胺
- 同双功能交联剂
- 高碘酸钠
- 流动相溶剂
- 固定化胰蛋白酶
Immobilized Pepsin resin (Pepsin Agarose) is commonly used to create F(ab)2 fragments from antibodies.By immobilizing pepsin autolysis and protease contamination is essentially eliminated.Immobilized Pepsin gel hydrolyzes protein non-specifically at pH 1.0 to 3.0, with an optimum activity at pH 1.0. ProteoChem"s highly active pepsin is covalently bound to crosslinked 6% beaded agarose resin and its specific activity is > 6,100 units/ml resin (one unit is defined as releasing 0.001 A280 as TCA hydrolyzed/min. at 37°C).ProteoChem"s Immobilized Pepsin is packaged as a slurry in buffer at pH 4.5 containing 50% glycerol, 100 mM Sodium Acetate, 0.05% NaN3.Pepsin protease cleaves non-specifically with some preference for tyrosines and phenylalanine (aromatic residues), although it generally does not cleave at alanine, glycine or valine residues. Recommended conditions are pH 1.0-4.5, 20°C to 37°C.
Click here for: Immobilized Pepsin Product Information Sheet & General Protocol
Special bulk pricing is available on bulk quantities of Immobilized Pepsin for your large volume manufacturing application.Available Options: 净化洗涤剂-净化洗涤剂解决方案ProteoChem的纯化洗涤剂水溶液具有超高纯度,低过氧化物含量和低羰基含量。纯化的聚山梨酯20,聚山梨酯80,聚乙二醇十二烷基醚,NP-40,Triton X-100和Triton X-114的10%(w / v)溶液可用于增溶膜蛋白,减少非特异性结合ELISA或IHC,有助于细胞膜裂解和其他蛋白质研究应用。纯化洗涤剂溶液的药物用途包括稳定乳液和悬浮液。ProteoChem的纯化表面活性剂溶液是最好的。它们的稳定性和纯度处于行业领先地位(<1.0 µeq / ml的过氧化物和羰基化合物)。
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