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商品描述
Specifications
SPECIESREACTIVITY: | Human |
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HOMOLOGY: | Predictedspeciesreactivitybasedonimmunogensequence:Bovine:(86%) |
TESTEDAPPLICATIONS: | ELISA,IF,IHC-P,WB |
APPLICATIONS: | ACE2antibodycanbeusedforthedetectionofACE2byWesternblotat1μg/mL.A90kDabandcanbedetected.Antibodycanalsobeusedforimmunohistochemistrystartingat2μg/mL.Forimmunofluorescencestartat10μg/mL. |
USERNOTE: | Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher. |
POSITIVECONTROL: | 1)Cat.No.1305-HumanKidneyTissueLysate |
SPECIFICITY: | Anti-ACE2hasnocrossresponsetoACE1. |
IMMUNOGEN: | ACE2antibodywasraisedagainstasyntheticpeptidecorrespondingtoaminoacidsneartheNterminusofhumanACE2. Theimmunogenislocatedwithinthefirst50aminoacidsofACE2. |
HOSTSPECIES: | Rabbit |
Properties
PURIFICATION: | ACE2Antibodyisaffinitychromatographypurifiedviapeptidecolumn. |
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PHYSICALSTATE: | Liquid |
BUFFER: | ACE2AntibodyissuppliedinPBScontaining0.02%sodiumazide. |
CONCENTRATION: | 1mg/mL |
STORAGECONDITIONS: | ACE2antibodycanbestoredat4˚Cforthreemonthsand-20˚C,stableforuptooneyear.Aswithallantibodiescareshouldbetakentoavoidrepeatedfreezethawcycles.Antibodiesshouldnotbeexposedtoprolongedhightemperatures. |
CLONALITY: | Polyclonal |
ISOTYPE: | IgG |
CONJUGATE: | Unconjugated |
AdditionalInfo
ALTERNATENAMES: | ACE2Antibody:ACEH,Angiotensin-convertingenzyme2,ACE-relatedcarboxypeptidase,ACEH |
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ACCESSIONNO.: | NP_068576 |
PROTEINGINO.: | 11225609 |
OFFICIALSYMBOL: | ACE2 |
GENEID: | 59272 |
Background
BACKGROUND: | ACE2Antibody:Angiotensin-convertingenzyme2(ACE2)playsacentralroleinvascular,renal,andmyocardialphysiology.IncontrasttoitshomologACE,ACE2expressionisrestrictedtoheart,kidney,andtestis.Recently.ACE2hasalsobeenshowntobeafunctionalreceptoroftheSARScoronavirus.ThenormalfunctionofACE2istoconverttheinactivevasoconstrictorangiotensinI(AngI)toAng1-9andtheactiveformAngIItoAng1-7,unlikeACE,whichconvertsAngItoAngII.Whiletheroleofthesevasoactivepeptidesisnotwellunderstood,lackofACE2expressioninace2-/ace2-miceleadstoseverelyreducedcardiaccontractility,indicatingitsimportanceinregulatingheartfunction. |
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REFERENCES: | 1)DonoghueM,HsiehF,BaronasE,etal.Anovelangiotensin-convertingenzyme-relatedcarboxypeptidase(ACE2)convertsangiotensionItoangiotension1-9.Circ.Res.2000;87:1-9. | 2)TipnisSR,HooperNM,HydeR,etal.Ahumanhomologofangiotensin-convertingenzyme.Cloningandfunctionalexpressionasacaptopril-insensitivecarboxypeptidase.JBiol.Chem.2000;275:33238-43. | 3)LiW,MooreMJ,VasilevaN,etal.Angiotensin-convertingenzyme2isafunctionalreceptorfortheSARScoronavirus.Nature2003;426:450-4. | 4)CrackowerMA,SaraoR,OuditGY,etal.Angiotensin-convertingenzyme2isanessentialregulatorofheartfunction.Nature2002;417:822-8. |