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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Specifications
| SPECIES: | Cynomolgus,Rhesusmacaque |
|---|---|
| SOURCESPECIES: | HEK293cells |
| SEQUENCE: | Val35-Asn242 |
| FUSIONTAG: | FcTag |
| TESTEDAPPLICATIONS: | ELISA,WB |
| APPLICATIONS: | ThisrecombinantproteincanbeusedforE,WB.Forresearchuseonly. |
| BIOLOGICALACTIVITY: | MeasuredbyitsbindingABIlityinafunctionalELISA.ImmobilizedCynomolgus/RhesusmacaqueCTLA-4,HisTagat2ug/mL(100uL/well)canbindCynomolgus/RhesusmacaqueB7-1,FcTagwithalinearrangeof1-8ng/mL(QCtested). |
Properties
| PURITY: | >95%asdeterminedbyreducedSDS-PAGE. Endotoxinlevelislessthan1.0EUperugbytheLALmethod. |
|---|---|
| PREDICTEDMOLECULARWEIGHT: | 50.5kDa |
| PHYSICALSTATE: | Lyophilized |
| BUFFER: | 50mMTris,100mMGlycine,pH7.5 |
| STORAGECONDITIONS: | LyophilizedProteinshouldbestoredat-20˚Corlowerforlongtermstorage.Uponreconstitution,workingaliquotsshouldbestoredat-20˚Cor-70˚C.Avoidrepeatedfreeze-thawcycles. |
AdditionalInfo
| ALTERNATENAMES: | CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7 |
|---|---|
| ACCESSIONNO.: | G7NXN7 |
Background
| B7-1andB7-2,togetherwiththeirreceptorsCD28andCTLA4,constituteoneofthedominantco-stimulatorypathwaysthatregulateTandBcellresponses.AlthoughbothCTLA4andCD28canbindtothesameligands,CTLA4bindstoB71andB72witha20100foldhigheraffinitythanCD28andisinvolvedinthedownregulationoftheimmuneresponse.B-lymphocyteactivationantigenB7-1(referredtoasB7)alsoknownasclusterofDifferentiation80(CD80),isamemberofcellsurfaceimmunoglobulinsuperfamilyandisexpressedonactivatedBcells,activatedTcells,macrophagesanddendriticcells.ItistheligandfortwodifferentproteinsontheTcellsurface:CD28(forautoregulationandintercellularassociation)andCTLA-4(forattenuationofregulationandcellulardisassociation).CD80worksintandemwithCD86toprimeTcells.CD80playsaroleininductionofinnateimmuneresponsesbyactivatingNF-kappaB-signalingpathwayinmacrophages.CD80isthusregardedaspromisingtherapeutictargetsforautoimmunediseasesandvariouscarcinomas. |


