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商品描述
Specifications
SPECIES: | Human |
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SOURCESPECIES: | CHOcells |
SEQUENCE: | Humanangiopoietin-1(aa20-498)isfusedattheN-terminustoaDDDDK-tag. |
FUSIONTAG: | DDDDKTag |
TESTEDAPPLICATIONS: | |
APPLICATIONS: | Thisrecombinantproteinsisforresearchuseonly. |
BIOLOGICALACTIVITY: | InducesphosphorylationofTie-2inprimaryculturedhumanvascularendothelialcells(HUVECs).Theproteinformsmainlytetramericstructures. |
Properties
PURITY: | >90%(SDS-PAGE) |
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PHYSICALSTATE: | Liquid |
BUFFER: | 0.2um-filteredsolutioninPBScontaining0.02%CHAPS. |
CONCENTRATION: | ~0.2mg/ml |
STORAGECONDITIONS: | Workingaliquotsarestableforupto3monthswhenstoredat-20˚C. |
AdditionalInfo
ALTERNATENAMES: | Ang-1,Ang1,Angpt1 |
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ACCESSIONNO.: | Q15389 |
PROTEINGINO.: | 20532340 |
Background
Angiopoietin-1(Ang-1)andangiopoietin-2(Ang-2)arecloselyrelatedsecretedligandswhichbindwithsimilaraffinitytoTie-2.Tie-2andangiopoietinshavebeenshowntoplaycriticalrolesinembryogenicangiogenesisandinmaintainingtheintegrityoftheadultvasculature.Ang-1activatesTie-2signalingonendothelialcellstopromotechemotaxis,cellsurvival,cellsprouting,vesselgrowthandstABIlization.Ang-2hasbeenidentifiedasasecretedproteinligandofTie-2andhasalternativelybeenreportedtobeanantagoNISTforAng-1inducedTie-2signalingaswellasanagonistforTie-2signaling,dependingonthecellcontext. |