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0512-67156496
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商品描述
Specifications
SPECIESREACTIVITY: | Human,Mouse,Rat |
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TESTEDAPPLICATIONS: | WB |
APPLICATIONS: | Westernblot:1-3ug/ml Providedassayconcentrationsaresuggestionsonly,14-3-3sigmaantibodytitrationmayberequiredforoptimalresults. |
USERNOTE: | Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher |
IMMUNOGEN: | Arecombinantproteinfragmentfromtheinternalregionof14-3-3sigmawasusedastheimmunogenforthisantibody.Theaminoacidsequenceiscommontothe~30kDaand~20kDaisoforms. |
HOSTSPECIES: | Rabbit |
Properties
PURIFICATION: | ProteinAaffinitychromatography |
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PHYSICALSTATE: | Liquid |
BUFFER: | PBSwith0.1mg/mlBSAand0.05%sodiumazide |
CONCENTRATION: | 0.5mg/mL |
STORAGECONDITIONS: | AliquotandStoreat-20C.Avoidfreez-thawcycles. |
CLONALITY: | Polyclonal |
ISOTYPE: | IgG |
CONJUGATE: | Unconjugated |
AdditionalInfo
ALTERNATENAMES: | 14-3-3proteinsigma,EpithelialcellMarkerprotein1,Stratifin,SFN,HME1 |
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OFFICIALSYMBOL: | SFN |
GENEID: | 2810 |
Background
BACKGROUND: | 14-3-3proteinsareregulatorsofadiversegroupofsignalingproteins,includingkinases,phosphatases,andtransmembranereceptors,andhaverolesinawiderangeofphysiologicalprocessesincludingtranscription,intracellularsignaling,cellcyclecontrolandapoptosis.Thereareseven14-3-3proteinsinmostmammals:beta,gammaepsilon,zeta,eta,sigmaandtau.14-3-3sigma,alsocalledStratifin,isaproductoftheSFNgene.ItisatumorsuppressorgeneinactivatedviaDNAmethylation.Reducedornoexpressionof14-3-3sigmaisseeninvariouscarcinomaincludingskin,breastandprostate,suggestingthat14-3-3genesilencingmaybeinvolvedintumorprogression. |
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