The Promokine Annexin V Apoptosis Detection Kit plus includes an additional dye, SYTOX green, that is impermeant to live and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with e.g. fluorescent Annexin V-Cy3 or Annexin V-PE conjugates and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. These populations can easily be distinguished by fluorescence microscopy using the appropriate filters.
PromoKine细胞生物学(Cell Biology)是在显微、亚显微和分子水平三个层次上,研究细胞的结构、功能和各种生命规律的一门科学。细胞生物学由细胞学发展而来,细胞学是关于细胞结构与功能(特别是染色体)的研究。现代细胞生物学从显微水平、超微水平和分子水平等不同层次研究细胞的结构、功能及生命活动。在我国基础学科发展规划中,细胞生物学与分子生物学、神经生物学和生态学并列为生命科学的四大基础学科。