Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels.
Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.
To view a list of references where this medium was used with other species/cell types please click here.
Components:
- Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix
- Growth Medium Kit: Includes Basal Medium and SupplementPack
- SupplementMix: Contains all media supplements premixed in one vial
- SupplementPack: Contains all media supplements as individual vials
Media supplement | Final supplement concentration |
---|---|
Fetal Calf Serum | 0.02 ml / ml |
Endothelial Cell Growth Supplement | 0.004 ml / ml |
Epidermal Growth Factor (recombinant human) | 0.1 ng / ml |
Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml |
Heparin | 90 μg / ml |
Hydrocortisone | 1 μg / ml |
Note: For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2. Heparin, included in all our other Endothelial Cell Growth Media formulations, may reduce transfection efficiency.
PromoKine细胞生物学(Cell Biology)是在显微、亚显微和分子水平三个层次上,研究细胞的结构、功能和各种生命规律的一门科学。细胞生物学由细胞学发展而来,细胞学是关于细胞结构与功能(特别是染色体)的研究。现代细胞生物学从显微水平、超微水平和分子水平等不同层次研究细胞的结构、功能及生命活动。在我国基础学科发展规划中,细胞生物学与分子生物学、神经生物学和生态学并列为生命科学的四大基础学科。