The anti-plakophilin 1 monoclonal antibody recognizes plakophilin 1 in stratified epithelia. Positive reaction with squamous cell carcinoma, negative with adenocarcinoma.

Host	mouse
Antibody Type	monoclonal
Isotype	IgG1
Clone	PP1-5C2
Immunogen	plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis)
Purification	hybridoma cell culture supernatant
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide
Storage	short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity	bovine, human, pig, Xenopus laevis

Tested applications	Tested dilutions
ELISA	assay dependent
Immunohistochemistry (IHC) - frozen	ready-to-use (preincubation with 0.05 - 0.2% Triton X-100, for 5-10 min recommended; for delicate tissues like liver and gastrointestinal material preincubation with saponin is preferred)
Immunohistochemistry (IHC) - paraffin	ready-to-use (pretreatment with 0.001% trypsin and microwave treatment recommended)
Western Blot (WB)	assay dependent

PROGEN 不会在内部饲养动物以产生抗体。PROGEN 的生产仅限于使用杂交瘤细胞培养技术产生的单克隆抗体。杂交瘤克隆获得德国学术合作伙伴的许可。多克隆抗血清从工业合作伙伴处获得，例如 Seramun Diagnostica GmbH。PROGEN 只选择严格遵守动物福利指导方针的合作者。PROGEN 大力支持动物福利工作，并积极参与创新方法的开发，以在不使用动物的情况下生成优质抗体。该公司已经提供了一种高效的噬菌体展示技术，用于生产重组、定制的抗体。这种方法的优势不仅在于避免使用动物，而且还可以生成定制的、可靠的抗体。