- Fast and easy recovery of DNA from agarose gel fragments
- High recovery of desired DNA
- Convenient spin column format
- DNA is ready for ligation, restriction digestion, sequencing and more
Figure 1.Efficient Recovery from Agarose Gels. The high yield of Norgen"s DNA Gel Extraction Kit is illustrated by purification of 1.5 µg of a 500 bp fragment from a 1% agarose gel using Norgen"s DNA Gel Extraction Kit and two other commercial kits.DNA recovery was quantified by running 300 ng (1/5th) of the input and 10 µL (1/5th) of the 50 µL DNA elution on a 1X TAE, 1% agarose gel followed by densitometry. The Norgen kit shows a significantly higher efficiency compared to the competitor kits based on the average of three purifications.
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Figure 1.Efficient Recovery from Agarose Gels
Figure 2.Efficient Recovery of Large DNAFragments. The efficient recovery of Norgen"s DNA Gel Extraction Kit is illustrated by purification of 1.5 µg of a 3,700 bp fragment from a 0.9% agarose gel using Norgen"s DNA Gel Extraction Kit (Lanes 5-7) and a competitor"s kit (Lanes 2-4).Eluted DNA was resolved on a 1X TAE, 0.9% agarose gel. Lane 1 indicates 300 ng (20%) of the input amount, while lanes 2-7 contain 10 µL (20%) of the 50 µL eluted amount.Norgen"s kit shows a higher recovery than the competitors kit.]">
Figure 2.Efficient Recovery of Large DNA Fragments
DNA Gel Extraction Kit
This kit is designed for the rapid preparation and purification of DNA fragments that have been fractionated on agarose gels. The recovered DNA is free from agarose and other impurities, and is compatible with restriction enzyme digestion, ligation into vectors and sequencing. The protocol can be completed in 20 minutes.
产品简介
品牌 其他品牌 供货周期 现货
应用领域 医疗卫生,食品,化工,生物产业,农业
北京百奥创新代理Norgen Biotek在中国的业务。Norgen Biotek全国代理,Norgen Biotek北京代理,Norgen Biotek华东代理,Norgen Biotek华北代理。详细介绍
RNA/Protein Purification Plus Kit
产品介绍
品牌:Norgen Biotek
货号:48200
中文名称:RNA/DNA/蛋白质纯化大提试剂盒(提取试剂盒)
该试剂盒提供了一种快速自旋柱方法,用于从培养的动物细胞、小组织样本、血液、细菌、酵母、真菌或植物的单个样本中分离和纯化总RNA、基因组DNA和蛋白质。该试剂盒采用两个柱:1)用于gDNA纯化,2)利用碳化硅柱进行RNA纯化(优于所有RNA大小的结合,包括miRNA)。蛋白质经RNA洗脱后在第二柱进行纯化。这些蛋白质在缓冲液中被洗脱,准备用于下游,不需要进一步的清洗。蛋白质可以直接定量,用于western blots, ELISA或质谱分析。