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蚂蚁淘在线 / 品牌中心 / Norgen Biotek / Norgen Biotek /PCR Purification Kit (Cat. 14400, 24800, 45700)/250preps/45700
商品描述
- Purify amplified DNA ranging from 100 bp -15,000 bp in size
- Fast and efficient spin column format
- Available in a 50 prep size and a 250 prep size
- Also available in 96 well format
Figure 1. High Yield Purification. The high yield of Norgen"s PCR Purification Kit is illustrated by purifying 3.5 µg of a 500 bp PCR-amplified product from a reaction mixture and comparing the recovery with a competitor.The purifications were performed in triplicate. DNA recovery was quantified by running 350 ng (1/10th) of the input and 5 µL (1/10th) of 50 µL DNA elution on a 1X TAE, 1% agarose gel followed by densitometry. The average yield for each kit is shown in Figure 1.Norgen"s kit shows a significantly higher recovery than the competitor"s kit.]">
Figure 1.High Yield Purification
Figure 2. Removal of Primer-Dimers. Primer-dimers present in PCR reactions are effectively removed during the purification process with Norgen"s PCR Purification Kit.One microgram of a 700 bp PCR fragment, spiked with 250 pmole of primer (Lane 1) was purified using Norgen"s kit, with high recovery and no traces of spiked primer present in the elutions (Lanes 2 and 3).Lane 1 contains 400 ng (40%) of the input, and Lanes 2 and 3 contain 20 µL (40%) of the 50 µL elution (duplicate).Lane M is Norgen"s MidRanger 1kb DNA Ladder.Eluted DNA was resolved on a 1X TAE, 1% agarose gel.]">
Figure 2.Removal of Primer-Dimers
PCR Purification Kit
This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
