Figure 2. Efficiency of the concentration of miRNA using Norgen"s Plant miRNA Purification Kit to detect three plant miRNAs. Small RNA was concentrated from 50 mg raspberry using Norgen"s Plant miRNA Purification Kit. Three µL of the elution was directly used for cDNA generation and 3 µL of cDNA was used for the CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad). Blue: concentrated small RNA from Norgen"s Plant miRNA Purification Kit, Red: Total RNA from Plant Total RNA Purification Kit (Cat. 25800), Circle: +RT, Cross: - RT.

]">

Figure 3. Isolation of Concentrated RNA that can be used in Sensitive Downstream Applications. Three different miRNA purification methods were compared for their ability to isolate and detect plant miRNA. miRNA was purified from grape using three miRNA purification kits according to the manufactures manual. Next, 3 µL of the elution was directly used for cDNA generation and 3 µL of cDNA was used for the CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) to detect the miRNA 159a. As it can be seen, only the miRNA purified using Norgen"s kit (blue lines) was amplified, while the miRNA isolated using the other 2 kits failed to amplify (red and green lines). Both of the competitors kits rely on phenol to isolate the miRNA, and therefore residual phenol is likely interfering with the amplification. Norgen"s kit isolates high yields of good quality miRNA without the use of phenol. Blue: Norgen Plant miRNA, Red: Competitor Q, Trizol: Green.

]">