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蚂蚁淘在线 / 品牌中心 / Norgen Biotek / Norgen Biotek /Total RNA Purification 96-Well Kit (Cat. 24300, 24350, 24370)/6x96-wellplates/24370
商品描述
- Extract high quality & purity total RNA including miRNA
- Convenient high throughput format for vacuum, centrifuge or robot
- Very consistent well-to-well RNA isolation
- No phenol step required - isolate all RNA in one fraction
- Bind & elute all RNA irrespective of size or GC content, without bias
- Isolate from a wide variety of specimens
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Figure 1. Consistent Yield of High Quality RNA with Complete Size Range without the Use of Phenol. Norgen"s Total RNA Purification 96-Well Kit allows for the consistent isolation of high quality RNA, with complete size range from the very large RNA down to small RNA without the use of phenol. Total RNA was isolated from 0.5 mL aliquots of an overnight culture of E. coli (~5 x 108 cells/mL) using Norgen"s Total RNA Purification 96-Well Kit in 16 replicates. Five microliters of the 75 µL isolated RNA was resolved on a 1.2% formaldehyde agarose gel. All 16 replicates showed both high yield and high quality. In addition, all replicates showed effective recovery of all sizes of RNA including the small RNA (arrow).]">
Total RNA Purification 96-Well Kit Figure 1
Figure 2. High Quality of RNA from a Diverse Range of Inputs. Norgen"s Total RNA Purification 96-Well Kit allows for RNA isolation from a wide range of speciesand tissue types. Total RNA was isolated from 8 mg of brain (Lanes A), kidney (Lanes B), liver (Lanes C), lung (Lanes D) and spleen tissue (Lanes E), 7.5 x 105 HeLa (Lanes F) and CHO cells (Lanes G), and 5 x 108 bacteria (Lanes H) using Norgen"s Total RNA Purification 96-Well Kit. Five microliters of the 75 µL isolated RNA was resolved on a 1.2% formaldehyde agarose gel. From observing the formaldehyde-agarose gel, it can be seen that Norgen"s kit can be used to successfully isolate total RNA, including small RNA species, from a broad range of sample types.]">
Total RNA Purification 96-Well Kit Figure 2
Figure 3. Isolation of Both Large and Small RNA. Norgen"s Total RNA Purification 96-Well Kitallows for consistent isolation of both large and small RNA. Total RNA was isolated from samples of 5 x 105 HeLa cells using Norgen"s Total RNA Purification 96-Well Kit. Aliquots of each total RNA sample were then used in 2 different RT-qPCR reactions. Ten microliters of the 75 µL isolated RNA was subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15 transcripts (Panel B), respectively. Both the mRNA and microRNA were amplified in a consistent manner from all the samples, with low variability of the Ct values. Thus both types of RNA are being consistently isolated using this kit.]">
Total RNA Purification 96-Well Kit Figure 3
Figure 4. Great Isolation Sensitivity. Norgen"s Total RNA Purification Kit allows for sensitive RNA extraction from even less than 10 cells. RT-qPCR was used to detect mRNA isolated from various input amounts of HeLa cells, from 100,000 down to a single cell, using Norgen"s Total RNA Purification 96-Well Kit. Ten microliters of the 75 µL isolated RNA was then subjected to a 20 µL reverse transcription using oligo dT primers. Three microliter of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human S15 transcripts. The resulting Ct values were plotted against the input cell number. Total RNA was isolated and detected linearly from as little as a single HeLa cell.]">
Total RNA Purification 96-Well Kit Figure 4
Total RNA Purification 96-Well Kit
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
The eluted RNA is consistent from well-to-well with high quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
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