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蚂蚁淘在线 / 品牌中心 / Norgen Biotek / Norgen Biotek /Plasmid MiniPrep Kit (Cat. 13300, 46400)/50preps/13300
商品描述
Plasmid MiniPrep Kit
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.
Figure 1.High DNA Yield from Small Cultures. Plasmid DNA from 1 mL overnight bacterial cultures was prepared with Norgens Plasmid MiniPrep Kit and compared with two other commercial kits.The purifications were performed in triplicate, and the average yield for each kit is shown in the graph. The quantification of the DNA yield was performed by resolving 5 µL of the 50 µL of eluted DNA on a 1X TAE, 0.9% agarose gel followed by densitometry. Norgen shows a significantly higher average yield than the other 2 competitors kits.]">
Figure 1.High Yield DNA from Small Cultures
Figure 2.High-Accuracy Sequencing. One microgram of plasmid DNA purified by Norgens Plasmid Miniprep DNA Kit was used as a template in an Applied Biosystem DNA Sequencer. The result showed an accuracy of >99% over a 950 bp contiguous sequence.]">
Figure 2.High Accuracy Sequencing
