FoodControl™LFAisaPCR-kitforfastandreliabledetectionoffoodbornepathogensviaconventionalPCRincombinationwithourreliableLateralFlowAssay(LFA)evaluationtechnology.
DetectingbacterialcontaminationisakeyaspectinfoodmicroBIOLOGy.Consequently,microbiologicalqualitycontrolprogramsareintensifiedthroughoutfoodchainproductioninordertominimizetheriskofintoxicatingconsumers.ReliABIlity,speedandrobustnessofatestsystemtodetectthepresenceorabsence,oreventhedegreeofcontaminationwithpathogens,becomesincreasinglyimportant.FoodControl™LFAisadiagnosticsystemforeasynon-quantitativedetectionofpresenceorabsenceoffoodbornepathogensinagriculturalorfoodindustryviaconventionalPCRandLateralFlowAssay(LFA)evaluationtechnology.
TypeofAssay
TheassayisbasedonaonetubeamplificationwithaconventionalPCRcyclerandasubsequentprobespecifichybridizationtechnique.Theresultinginterpretationisasimplereadoutofcolouredbarsonalateralflowstripe.
RecommendedUse/Scope
Canbeusedinresearchandindustryfordetectionofpathogeniccontaminationinfoodoragriculturalproducts.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.
ThekitisavailableforthedetectionofthefollowingmicroorganismsviaconventionalPCR:
Features
Micro-organism | Target |
Salmonellaenterica | invasionproteinA(invA)gen |
Yersiniaenterocolitica | heatstableenterotoxinAgen |
Shigellaspp. | invasionplasmidantigen(ipaH6)gen |
Campylobacterspp. | acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase(lpxA)gen |
Clostridiumperfringens | phospholipaseCalphatoxin(plc)gen |
Shigelladysenteriae, Escherichiacoli(EHEC) | ShigaToxin1(stx1)gen |
Shigelladysenteriae, Escherichiacoli(EHEC) | ShigaToxin2(stx2)gen |
EscherichiacoliO157 | wBDRgen |
EscherichiacoliO104 | wckDgen |
Listeriaspp. | invasionassociatedproteinp60(iap)gen |
Listeriamonocytogenes | listeriolysinO(hly)gen |
Salmonellaspp. | spacer-regionbetween16Sand23SRNAgenes |