MeatID™Screenisakitforthefastandreliableidentificationofseveralanimalspecies(Goat,Sheep,Pork,Horse,Beef,Donkey,Fish,Chicken,TurkeyandCamel)inmeatbyqPCRinasingleparallelrun.
Theidentificationofdifferentmeats,especiallyinmincedmeatproductsisaserioustaskinfoodsafetyandfromandethicalperspective,especiallyforMuslims.Authenticationofforbiddenornonedeclaredingredientssuchasporkorsubstandardmeatisessentialtoensureconfidenceinthesupplychainandregulatorycompliance.
MeatID™Screenisavailableforrapidandreliableanalysisfromvariousmatricesincludingraw,orevenhighlyprocessedandcookedmeatproductswheretheDNAmaybesignificantlydegraded.ItispossIBLetoidentifyrelevantspeciesdowntoathresholdlevelof0.5%withasemi-quantitativeresult.
TypeofPCR
TaqMan®AssaybasedonFAM™andHEX™labeledprobes.
RecommendedUse/Scope
Canbeusedinresearchandindustryfordetectionofanimalspecies(Goat,Sheep,Pork,Horse,Beef,Donkey,Fish,Chicken,Turkey,Camel,Zebra)inmeatandotherfoodsbyqPCR.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.
Targetsequenceisamitochondrialgene(cytochromeb),whichisavailableinmultiplecopiespercell,enablingsuccessfuldetectionofminuteamountsoftargetDNA!
PackageSizes
- Cat.No.12-01-0055tests
Cat.No.12-01-02020tests
Cat.No.12-01-04040tests
ResultEvaluation
cyclerbased,real-timePCR
Sensitivity
Downtoathresholdlevelof0.5%withasemi-quantitativeresult.
Content
Primersets,nucleotides,polymeraseandinternalcontrolDNA,lyophilized,filledin0.2mlPCRreactiontubes(Stripes).Rehydrationbuffer,Positivecontrol,PCRgradewater.
SampleRequirements
DNAcanbeextractedfromsamplematerialusingDNAextractionkitse.g.ExtractNow™DNAMiniKit(Art.-Nr.608-1050)orusermethod.
RequiredConsumables
Pipettetips,1.5mlreactiontubes
RequiredLabDevices
qPCRcyclerwithFAM™andHEX™filter,benchtopcentrifuge,racksfortubes,variablemicroliterpipettes,sterilefiltertips
Timetoresult
approx.90minutes
ShelfLifeandStorage
Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydrationreagentsmustbestoredat-18°C.
Fig.1:SensitivityoftheMeatID™Screen,parameterHorse.
Fig.2:Detectionof1%w/whorsemeatinrespectivemeatbackground