ValidationtoolsforconventionalPCRcycler
ValidationtoolsfortestingPCRthermalcyclersaccordingtolegalrequirements
ISO17025,EN45001,ISO13485,ISO/TS20836:2007,GLP,GMP,IQ/OQ/PQ,andothers.
CyclerCheckKits
ThePCRCyclerCheckKitsareavailableforbothconventionalendpointPCRandreal-timePCR(qPCR)cyclers.
FalsenegativePCRresultsarehighlycriticalandmightbecausedbyadefectPCRcycler.Verificationofthecorrecttemperaturecontroloftheusedequipmentisusuallyastressfulissue.TocomplywithqualitymanagementsystemsisnoteasytofulfillforPCRcyclers.
Principle
ThePCRCyclerCheckKitsprovidetemperaturesensitivePCRreactionstomonitoranupperandlowertemperaturerangeinonerun.Theprimer/probesequencesinthePCRCyclerCheckKitsweredesignedtoreactextremelysensitivetoincorrecttemperaturecontrol,temperaturehomogeneity,precisionandtiming.Amplificationwillcompletelydropoutattemperaturedifferencesofmorethan2°C.ThecyclerperformanceistestedatstandardPCRsettingstoreflectmostuser’sapplications.Inaddition,thepre-adjustedtargetconcentrationsareonlyamplifiedathighPCRefficienciesasanadditionalindicatorforaccuratetemperaturecontrolofthethermalcycler.
Benefits
•Noextraequipment,instrumentorsoftwareneeded.
•ApplicablewithanyblockPCRcyclerorqPCRcyclerusedinaresearchorindustrialqualityassurancelabforthereliABIlitytestingofinstrumentperformanceandreliability.
•IntheqPCRThermalCyclerValidiationKittheprobesofeachamplificationsystemarelabeledwithdifferentfluorescentdyes(FAM™andROX™)toallowindividualevaluationofeachtemperaturerange.
•Includesallyouneed:Freeze-driedprimer/probesets,polymerase,nucleotidesandrehydrationbuffer
- Cat.No.57-2102PCRCyclerCheck™Advance 6strips,8vialseach
Cat.No.57-2103PCRCyclerCheck™OneStep 100reactions