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商品描述
HighpurityAdenosine5’-triphosphateforuseasacofactorforresearch,biochemicalenzymeassaysand invitro diagnosticanalysis.
Adenosine5’-triphosphate,disodiumsalt(ATP,5’-ATP,ATP-Na2).MW:507.18forfreeacid.Purity:99.3%;ATPcontent: 85.4%;sodiumcontent: 7.5%;moisturecontent: 6.5%.
DatabookletsforeachpacksizearelocatedintheDocumentationtab.
MethodfortheDirectDeterminationofAvailableCarbohydratesinLow-CarbohydrateProductsUsingHigh-PerformanceAnionExchangeChromatography.
Ellingson,D.,Potts,B.,Anderson,P.,Burkhardt,G.,Ellefson,W.,Sullivan,D.,Jacobs,W.&Ragan,R.(2010).JournalofAOACInternational,93(6),1897-1904.
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Animprovedmethodfordirectdeterminationofavailablecarbohydratesinlow-levelproductshasbeendevelopedandvalidatedforalow-carbohydratesoyinfantformula.Themethodinvolvesmodificationofanexistingdirectdeterminationmethodtoimprovespecificity,accuracy,detectionlevels,andruntimesthroughamoreextensiveenzymaticdigestiontocaptureallavailable(orpotentiallyavailable)carbohydrates.Thedigestionhydrolyzesallcommonsugars,starch,andstarchderivativesdowntotheirmonosaccharidecomponents,glucose,fructose,andgalactose,whicharethenquantitatedbyhigh-performanceanion-exchangechromatographywithphotodiodearraydetection.Methodvalidationconsistedofspecificitytestingand10daysofanalyzingvariousspikelevelsofmixedsugars,maltodextrin,andcornstarch.TheoverallRSDwas4.0acrossallsampletypes,whichcontainedwithin-dayandday-to-daycomponentsof3.6and3.4,respectively.Overallaveragerecoverywas99.4(n=10).Averagerecoveryforindividualspikedsamplesrangedfrom94.1to106(n=10).Itisexpectedthatthemethodcouldbeappliedtoavarietyoflow-carbohydratefoodsandbeverages.
DeterminationofFreeandTotalMyo-InositolinInfantFormulaandAdult/PediatricNutritionalFormulabyHigh-PerformanceAnionExchangeChromatographywithPulsedAmperometricDetection.
Ellingson,D.,Pritchard,T.,Foy,P.,King,K.,Mitchell,B.,Austad,J.,Winters,D.,Sullivan,D.&Dowell,D.(2013).JournalofAOACInternational,96(5),1068-1072.
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DeterminationofFreeandTotalMyo-InositolinInfantFormulaandAdult/PediatricNutritionalFormulabyHigh-PerformanceAnionExchangeChromatographywithPulsedAmperometricDetection,IncludingaNovelTotalExtractionUsingMicrowave-AssistedAcidHydrolysisandEnzymaticTreatment:FirstAction2012.12.Afteranassessmentofdatageneratedfromasingle-laboratoryvalidationstudypublishedinJ.AOACInt.95,1469–1478(2012),amethodfordeterminingtotalmyo-inositolininfantformulaandadult/pediatricnutritionalformulabyhigh-performanceanionexchangechromatographywithpulsedamperometricdetection(HPAEC-PAD),includingextractionbyusingmicrowave-assistedacidhydrolysisandenzymatictreatmentwaspresentedforconsiderationbyAOACduringtheAOACAnnualMeetingheldinLasVegas,NV,fromSeptember30toOctober3,2012.TheExpertReviewPanelonInfantFormulaandAdultNutritionalsconcludedthatthemethodmetthecriteriasetbythestandardmethodperformancerequirements(SMPRs)forthedeterminationoffreemyo-inositolandapprovedthemethodasAOACOfficialFirstAction.Themethodalsodeterminestotalmyo-inositol,butincludesboundsourcesthattheSMPRsexclude.ThemethodinvolvesusingHPAEC-PADforfreemyo-inositolandatotalmyo-inositoldeterminationbytwodifferenttechniques.Thefirsttechniqueusestheconventionalacidhydrolysiswith6hincubationinanautoclave.Thesecondusesamicrowave-assistedacidhydrolysiswithenzymatictreatmentthatdecreasestheextractiontime.
