Highpurityrecombinantendo-α-N-Acetylgalactosaminidase(Enterococcusfaecalis)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
EC3.2.1.97
CAZyFamily:GH101
CAS:59793-96-3
endo-alpha-N-acetylgalactosaminidase;glycopeptide-D-galactosyl-N-acetyl-alpha-D-galactosamineD-galactosyl-N-acetyl-galactosaminohydrolase
Recombinant.FromEnterococcusfaecalis.
In20mMTris.HClpH7.5,50mMNaCl,5mMEDTA.
Suppliedat~1U/mL.
Specificactivity:
~2U/mg(37oC,pH7.5onGal-β-1,3-GalNAc-α-pNP).
StABIlity:>2yearsat-20oC.
Customquantitiesandformulationsavailableuponrequest.
DESCRIPTION
endo-α-N-Acetylgalactosaminidase(Enterococcusfaecalis)
EC3.2.1.97
CAZyFamily:GH101
CAS:59793-96-3
Synonyms:
endo-alpha-N-acetylgalactosaminidase;glycopeptide-D-galactosyl-N-acetyl-alpha-D-galactosamineD-galactosyl-N-acetyl-galactosaminohydrolase
Form:
In20mMTris.HClpH7.5,50mMNaCl,5mMEDTA.
Stability:
>2yearsat-20oC.
Specificactivity:
~2U/mg(37oC,pH7.5onGal-β-1,3-GalNAc-α-pNP).
Unitdefinition:
OneUnitofendo-α-N-acetylgalactosaminidaseactivityisdefinedastheamountofenzymerequiredtoreleaseoneµmoleofp-nitrophenolperminutefromGal-β1,3-GalNAc-α-pNP(0.2mM)inTris.HClbuffer(100mM)pH7.5at37oC.
Specificity:
HydrolysisoftheO-glycosidic-α-linkagebetweenGal-β-1,3-GalNAc(Core1)orGlcNAc-β-1,3GalNAc(Core3)andaserineorthreonineresidueofglycopeptidesandglycoproteins.
Applications:
Applicationsinclinicalchemistry,glycoBIOLOGyandcarbohydrateresearch.
