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Medchemexpress/MTT(Synonyms: Thiazolyl Blue; Thiazolyl Blue Tetrazolium Bromide; Methylthiazolyldiphenyl-tetrazolium bromide)

价格
¥1100.00
货号:HY-15924-10mM*1mLinDMSO
浏览量:106
品牌:MCE
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商品描述
MTT,ayellowtetrazole,isreducedtopurpleformazaninlivingcells,andusedforassessingcellmetabolicactivity.
Description

MTT,ayellowtetrazole,isreducedtopurpleformazaninlivingcells,andusedforassessingcellmetabolicactivity.

InVitro

MTTisusedinconjunctionwithrhodamineBtomeasuremitochondrialmembranepotential.MTT-formazanisgeneratedinmitochondriaactsasafluorescencequencherforrhodaminethatdistributesacrossmembranesofviablecellsaccordingtomembranepotential.IntheabsenceofmPMS,cellularreductionofMTTisstrong.MTTismembrane-impermeablewhenincorporatedintolargeunilamellarliposomes,andthereforeitistakenupbycellsviaendocytosis[1].

ClinicalTrial
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References
  • [1].BerridgeMV,etal.TetrazoliumdyesastoolsincellBIOLOGy:newinsightsintotheircellularreduction.BiotechnolAnnuRev.2005;11:127-52.

PreparingStockSolutions
ConcentrationVolumeMass1mg5mg10mg
1mM2.4136mL12.0680mL24.1359mL
5mM0.4827mL2.4136mL4.8272mL
10mM0.2414mL1.2068mL2.4136mL
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.
References
  • [1].BerridgeMV,etal.Tetrazoliumdyesastoolsincellbiology:newinsightsintotheircellularreduction.BiotechnolAnnuRev.2005;11:127-52.

MolecularWeight

414.32

Formula

C₁₈H₁₆BrN₅S

CASNo.

298-93-1

Storage
Powder-20°C3years
 4°C2years
Insolvent-80°C6months
 -20°C1month
Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

DMSO:≥32mg/mL;H2O:0.88mg/mL(Needultrasonicandwarming)

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:99.59%