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蚂蚁淘在线 / 品牌中心 / MCE / Medchemexpress/D-Luciferin(Synonyms: D-(-)-Luciferin; Firefly luciferin)/HY-12591A/50mg

Medchemexpress/D-Luciferin(Synonyms: D-(-)-Luciferin; Firefly luciferin)/HY-12591A/50mg

价格
￥1100.00
货号：HY-12591A-10mM*1mLinDMSO
浏览量：108
品牌：MCE
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商品描述
D-luciferinisthenaturalsubstrateofluciferasesthatcatalyzetheproductionoflightinbioluminescentinsects.
Description

D-luciferinisthenaturalsubstrateofluciferasesthatcatalyzetheproductionoflightinbioluminescentinsects.

InVitro

D-luciferinisthenaturalsubstrateoftheenzymeluciferase(Luc),thatcatalyzestheproductionofthetypicalyellowgreenlightoffireflies.ThepresentreviewcoversthesynthesisofD-luciferinandderivativesoranaloguesthataresubstratesorinhibitorsoftheluciferasefromtheAmericanfireflyPhotinuspyralis,theenzymemorefrequentlyusedintechniquesofinvitroandopticalimaging[1].

InVivo

Bioluminescenceimaging(BLI)usingthefireflyluciferase(Fluc)asareportergeneandD-luciferinasasubstrateiscurrentlythemostwidelyemployedtechnique.ThetotalsignalintensityisplottedagainstthetimeafterD-luciferininjectiontogenerateatime-intensitycurve.Inadditiontothepeaksignal,thesignalsatfixedtimepoints(5,10,15,and20 min)afterD-luciferininjectionaredeterminedasalternativestothepeaksignal.Thesignalinagiventime-intensitycurveisnormalizedforthepeaksignalinthecurvetorepresentthepatternoftemporalchangesafterD-luciferininjection[2].

References

  • [1].GiuseppeMeroni,etal.D-Luciferin,derivativesandanalogues:synthesisandinvitro/invivoluciferase-catalyzedbioluminescentactivity.ARKIVOC2009(i)265-288.

    [2].InoueY,etal.Timingofimagingafterd-luciferininjectionaffectsthelongitudinalassessmentoftumorgrowthusinginvivobioluminescenceimaging.IntJBiomedImaging.2010;2010:471408.

PreparingStockSolutions
ConcentrationVolumeMass1mg5mg10mg
1mM3.5674mL17.8368mL35.6735mL
5mM0.7135mL3.5674mL7.1347mL
10mM0.3567mL1.7837mL3.5674mL
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.
AnimalAdmiNISTration
[2]

D-luciferinispreparedinphosphate-bufferedsaline(Mice)[2].

Mice[2]
InvivoBLIisperformedusingacooledcharge-coupleddevicecamerasystem(IVISImagingSystem100)3,5,7,10,12,14,19,21,24,and28daysaftertheinoculationofHCT116-Luccells.Miceareinjectedwith75 mg/kgD-luciferinin100 Lofphosphate-bufferedsalinesubcutaneouslynearthescapulaandwereplacedinthelight-tightchamberoftheimagingsystemunderisofluraneanesthesia.Beginning5 minafterinjection,dorsalluminescentimageswithanexposuretimeof1 sareacquiredsequentiallyatarateofoneimageperminuntil20 minafterD-luciferininjection.Dataacquisitioniscontinueduntil40 minpostinjectionondays3or5anduntil25 minonday7,becauseoftheprolongedtimecourseoflightemission.Binningis4andthefieldofviewis15 cm.
MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

References

  • [1].GiuseppeMeroni,etal.D-Luciferin,derivativesandanalogues:synthesisandinvitro/invivoluciferase-catalyzedbioluminescentactivity.ARKIVOC2009(i)265-288.

    [2].InoueY,etal.Timingofimagingafterd-luciferininjectionaffectsthelongitudinalassessmentoftumorgrowthusinginvivobioluminescenceimaging.IntJBiomedImaging.2010;2010:471408.

MolecularWeight

280.32

Formula

C₁₁H₈N₂O₃S₂

CASNo.

2591-17-5

Storage
Powder-20°C3years
 4°C2years
Insolvent-80°C6months
 -20°C1month
Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

DMSO:14.7mg/mL

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:98.11%