Pepstatin is a potent inhibitor of aspartyl proteases, and inhibits the aspartic proteases cathepsin D, pepsin, and renin.
Description
Pepstatin is a potent inhibitor of aspartyl proteases, and inhibits the aspartic proteases cathepsin D, pepsin, and renin.
In Vivo
A low dose of pepstatin shows an inhibition of edema formation, and intraperitoneal injection of 1.25 mg/kg of pepstatin causes a 30% inhibition with a a very low toxicity[1]. Pepstatin increases LC3-II levels, while it is reverted by 3-methyladenine (3-MA)[2].
References
[1]. Umezawa H, et al. Pepstatin, a new pepsin inhibitor produced by Actinomycetes. J Antibiot (Tokyo). 1970 May;23(5):259-62.
[2]. Raquel T. Lima, et al. Modulation of Autophagy by a Thioxanthone Decreases the Viability of Melanoma Cells. Molecules 2016, 21(10), 1343
Preparing Stock Solutions
ConcentrationVolumeMass
1 mg
5 mg
10 mg
1 mM
1.4580 mL
7.2898 mL
14.5796 mL
5 mM
0.2916 mL
1.4580 mL
2.9159 mL
10 mM
0.1458 mL
0.7290 mL
1.4580 mL
Please refer to the solubility information to select the appropriate solvent.
References
[1]. Umezawa H, et al. Pepstatin, a new pepsin inhibitor produced by Actinomycetes. J Antibiot (Tokyo). 1970 May;23(5):259-62.
[2]. Raquel T. Lima, et al. Modulation of Autophagy by a Thioxanthone Decreases the Viability of Melanoma Cells. Molecules 2016, 21(10), 1343
Molecular Weight
685.89
Formula
C₃₄H₆₃N₅O₉
CAS No.
26305-03-3
Storage
Powder
-80°C
2 years
-20°C
1 year
In solvent
-80°C
6 months
-20°C
1 month
Shipping
Room temperature in continental US; may vary elsewhere
