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- •New J Chem. 18 Oct 2017.
| Description |
JC-1, a fluorescent lipophilic carbocyanine dye, that can be used to measure mitochondrial membrane potential. |
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| In Vitro |
JC-1 (2.5 μM) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers[1]. Fluorescent labeling of mitochondria with either JC-1 (1 μg/mL, 15 min), reveals that are distributed irregularly, resulting in regions of high and low mitochondrial content within astrocytes[2]. JC-1 has been shown to interact with α-synuclein at the acidic C-terminal region with a Kd of 2.6 μM. JC-1 itself does not accelerate the protein aggregation of α-synuclein in the absence of iron, insted, it decelerates the aggregation process by extending the lag phase approx[3]. JC-1 is avidly accumulated in sensitive K562 cells where it displays both a green cytoplasmic and red mitochondrial fluorescence. JC-1 is poorly accumulated in resistant K562 cells, which displays only a slight green fluorescence[4]. |
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| Preparing Stock Solutions |
Please refer to the solubility information to select the appropriate solvent.
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| References |
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| Molecular Weight |
652.23 |
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| Formula |
C₂₅H₂₇Cl₄IN₄ |
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| CAS No. |
3520-43-2 |
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| Storage |
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| Shipping | Room temperature in continental US; may vary elsewhere |
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| Solvent & Solubility |
10 mM in DMSO
* "<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation=""> |
Purity: >95.0%
COA (94 KB) HNMR (449 KB) LCMS (187 KB)
Handling Instructions (1252 KB)-
[1]. A Perelman, et al. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 2012 Nov 22;3:e430.
[2]. Vera C. Keil, et al. Ratiometric high-resolution imaging of JC-1 fluorescence reveals the subcellular heterogeneity of astrocytic mitochondria. Pflügers Archiv - European Journal of Physiology. 2011,462(5): 693-708.
[3]. Jung-Ho LEE, In-Hwan LEE, Young-Jun CHOE, et al. Real-time analysis of amyloid fibril formation of α-synuclein using a fibrillation-state-specific fluorescent probe of JC-1. Biochem. J. 2009, 418:311-323.
[4]. Salvioli S, et al. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 1997 Jul 7;411(1):77-82.
