Description: T4DNAPolymerasecatalyzestheextensionofaprimedDNAtemplateinthe5'->3'direction.Thisenzymeexhibitsapowerful3'->5'exonucleaseactivity,whilelackinganyinherent5'->3'exonucleaseorstranddisplacementfunctions.
SpecificActivity:5,555U/mg
Application: -3'-overhangremovaltoformbluntends -5'-overhangfill-intoformbluntends -Singlestranddeletionforsub-cloning -Secondstrandsynthesisinsite-directedmutagenesis -Probelabelingusingreplacementsynthesis
Source: PurifiedfromastrainofE.colithatexpressestherecombinantT4DNAPolymerasegene.
Suppliedin: 100mMKPO4 1.0mMDTT 0.1mMEDTA 50%Glycerol pH6.5@25°C
SuppliedWith:10xBlueBuffer
10xBlueBuffer: 500mMNaCl 100mMTris-HCl 100mMMgCl2 10mMDTT pH7.9@25°C
UnitDefinition: Oneunitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPsintoacid-precipitablematerialin30minutesat37°C.
RecommendedStorageCondition:-20ºC
Reference: 1.Tabor,S.andStruhl,K.(1989)InDNA-DependentDNAPolymerases.F.M.Ausebel,R.Brent,R.E.Kingston,D.D.Moore,J.G.Seidman,J.A.SmithandK.Struhl(Eds.),CurrentProtocolsinMolecularBIOLOGy,pp.3.5.10-3.5.12.2.Sambrook,J.,Fritsch,E.F.andManiatis,T.(1989)MolecularCloning:ALaboratoryManual,(2ndEd.),5.44-5.47. 2.Dale,R.,McClure,B.andHouchins,J.(1985)Plasmid,13,31-40. 3.Kunkel,T.A.,Roberts,J.D.andZakour,R.A.(1987)R.WuandL.Grossman(Eds.),MethodsEnzymol.,154,pp.367-382.SanDiego:AcademicPress. 4.Panet,A.,vandeSande,J.H.,Loewen,P.C.andKhorana,H.G.(1973)Biochemistry,12,5045-5050. |