HydrogenPeroxideDetection
lumigenHyPerBluChemiluminescentReagentisanovelready-to-usesubstrateforthedirectdetectionofhydrogenperoxide.Reactionofthesubstratewithhydrogenperoxiderapidlygeneratessustainedhigh-intensityluminescenceformaximumdetectionsensitivityinsolutionassays.CoupledwithoxidasestheLumigenHyPerBlusubstratealsoallowsonestepindirectquantitationofoxidasesubstratesortheoxidaseitself.
LumigenHyPerBlusubstrateissuitableforautomationandminiaturizationto384-wellor1536-wellformat.LumigenHyPerBlureagentswidetoleranceforcommonassayadditives(egDMSO),lackofintermediaries,chemicalspecificity,one-componentstructureandexcellentstABIlityleadtoassaysimplicity,excellentrobustnessandunmatchedconvenienceforhighthroughputscreeninglaboratories.
- Directdetectionofperoxide,oxidaseenzymesortheirsubstrates
- Singleready-to-usereagentformulationwithlongstabilityforconvenience
- Broaddynamicrangewithbrightsustainedchemiluminescence
- Excellentsensitivityinsolutionassays
LumigenHyPerBluTechnology
LumigenHyPerBlureagentisanovelchemiluminescentsubstratethatdirectlyreactswithhydrogenperoxide(withoutperoxidase)rapidlygeneratingasustainedhigh-intensitychemiluminescence.ThesignalintensitycanbemeasuredonPMTbasedplatereADIngluminometersorCCDimagingsystems.Longstabilityoftheformulationcombinedwiththewidehydrogenperoxidedynamicrange(>5orders)allowforsensitiveandreproducIBLeassays.
IndirectDetectionofOxidases
LumigenHyPerBluchemiluminescentreagentoffersano-labelenzyme-freetechniqueforthedetectionofoxidaseactivity.BecausetheLumigenHyPerBlusubstratedirectlymeasureshydrogenperoxideproducedfromoxidativereactions,assaysaresignificantlysimpler,moreflexible,andavoidcomplicationsassociatedwithlabel-basedtechnologies.