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蚂蚁淘在线 / 品牌中心 / lumigen / lumigen/Lumi-Phos Plus/P-701/100 mL

lumigen/Lumi-Phos Plus/P-701/100 mL

价格
面议
货号：P-701
浏览量：127
品牌：lumigen
服务
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商品描述

BlottingAPSubstrate

Lumi-PhosPlusisanovel,ready-to-usechemiluminescentreagentformulationcontainingthealkalinephosphatase(AP)substrate,lumigenPPDandapatentedenhancer.Lumi-PhosPlusprovideshighlysensitivechemiluminescentdetectionofAPwithDNAprobesorantibodyconjugatesinsolutionandonsolidsupportssuchasmembranes.Thisreagentisrecommendedforeitherdirectchemiluminescentdetectionofalkalinephosphatase(AP)labelednucleicacidsorindirectlytodetecthapten-labelednucleicacidsprobedwithspecificanti-haptenantibodiesconjugatedwithAPonaSouthernblotmembraneoranyothersolidsurface.

  • Excellentsensitivity–singlecopygenedetection
  • Highintensitylightproductionwithlowbackground
  • Sustainedluminescence-withlowconcentrationsofAP,thelightintensityremainsconstantforseveralhours
  • HighstABIlityandlongshelflife

SolutionKinetics

Lumi-PhosPlushasasignificantlyhighersignaltobackgroundratiothanLumi-Phos530duetoitslowerreagentbackground.Lumi-PhosPlusreaches90%ofmaximumlightoutputapproximately27minutesafterintroductionofenzymeinhomogeneousassaysat37°C.Lightemissionismaximalat470nm.

Blotting AP Substrate

Lumi-Phos Plus

ProductSpecifications

Enzyme: 
AlkalinePhosphatase(AP)
Application: 
SouthernandNorthernBlotting,DotBlots,CDNAArrays,ColonyandPlaqueProbing
Sensitivity: 
SinglecopygenedetectionongenomicSouthernblot
StorageConditions: 
2-8°C;Storeinamberbottletoprotectfromlight
Stability: 
2yearsstoredat2-8°C
WorkingSolution: 
Readytousesolution
umigen PPD Lumigen® PPD (4-methoxy-4-(3-phosphatephenyl)spiro [1,2-dioxetane-3,2'-adamantane], disodium salt) is used for ultrasensitive chemiluminescent detection of alkaline phosphatase in immunoassays and DNA probe assays using magnetic particles, beads, tubes and microtiter plates as well as nylon and PVDF membranes.